EGFR、COX-2及P63蛋白表达与甲状腺乳头状癌侵袭转移的关系

目的　探讨甲状腺乳头状癌 (PTC)中表皮生长因子受体 (EGFR)、环氧化酶 2 (COX 2 )和 p6 3蛋白的表达与肿瘤侵袭转移的关系。方法　采用免疫组化S P法检测 6 7例PTC(其中腺内侵袭 4 1例、腺外侵袭 2 6例 ;有淋巴结转移 2 9例、无淋巴结转移 38例 )、3例滤泡癌和 15例甲状腺腺瘤中EGFR、COX 2及 p6 3蛋白的表达。结果　EGFR、COX 2和 p6 3蛋白在PTC组织中的阳性表达率分别为 88 1%、82 1%和 70 2 % ,均显著高于甲状腺腺瘤 (P <0 0 5 ) ;EGFR与COX 2、COX 2与p6 3蛋白在PTC组织中的的表达呈明显正相关 (P <0 0 5 ) ;EGFR和COX 2在PTC腺外侵袭组和有淋巴结转移组阳性表达率均明显高于腺内侵袭组和无淋巴结转移组 (P <0 0 5 ) ,p6 3蛋白阳性表达率与淋巴结转移有关 (P <0 0 5 )、与浸润程度无关。结论　EGFR与COX 2在PTC组织中的高表达可能促进肿瘤的侵袭和转移 ,p6 3蛋白在PTC的高表达提示其与PTC的细胞增殖相关

Relationship between the expression of epidermal growth factor receptor, cyciooxygenase-2, p63 protein and invasion, metastasis of human papillary thyroid carcinoma

Purpose To study the relationship between the expression of epidermal growth factor receptor (EGFR), cyclooxygenase-2(COX-2) and p63 protein and invasion,metastasis of human papillary thyroid carcinoma(PTC). Methods The expression of EGFR,COX-2 and p63 protein was determined with S-P immunohistochemical analysis in 67 PTCs(including 41 cases with intrathyroidal invasion and 26 cases with extrathyroidal invasion,29 cases with lymph node metastasis and 38 cases without lymph node metastasis),3 follicular carcinomas and 15 thyroidal adenomas. Result Positive rates of EGFR(88.1%),COX-2(82.1%) and p63 protein(70.2%) in PTC were significantly higher than those in adenomas(P<0.05). There were positive correlations between the expression of EGFR and COX-2(P<0.01), COX-2 and p63 protein(P<0.05). Overexpression of EGFR and COX-2 in PTC was related to extrathyroidal invasion and lymph node metastasis(P<0.05). Overexpression of p63 protein was related to lymph node metastasis(P<0.05). Conclusions In PTC, the EGFR and COX-2 may play an important role in papillary thyroid cancer progression. In PTC, the expression of p63 protein indicates the correlation between the p63 protein and cell proliferation.