经大鼠脾动脉行肝细胞移植建立动物模型

 目的 建立经SD大鼠颈动脉途径脾动脉插管行肝细胞移植的动物模型,并探讨其临床意义。方法 采用D-氨基半乳糖(D-galactosamine,D-gal)诱导大鼠急性肝损伤(acute liver injury,ALI),24 h后40只SD大鼠分为两组:A组(n=20)经左侧颈动脉插管,进入腹腔干,通过脾动脉移植1×107个肝细胞;B组(n=20)通过同样的方法经脾动脉注射0.5 mL Hank′s液作为对照,观察不同时间点（1、3、6、10、15天）大鼠肝功能的变化情况以及脾内白蛋白合成作用,HE染色观察脾内肝细胞分布情况。结果 经颈动脉途径脾动脉插管成功率约90%,荧光示踪剂CFDA SE标记的肝细胞脾内移植1天后,受体大鼠脾脏可以看到散在绿色荧光分布。A组移植10天后,HE染色可以看到肝细胞在脾脏散在分布;A组移植15天后,共聚焦白蛋白免疫荧光结果表明,脾内有白蛋白绿色荧光信号。结论 经大鼠颈动脉途径脾动脉插管移植的肝细胞能在脾内存活,证实该途径是肝细胞有效移植途径之一。

Establishment of hepatocyte transplantation model via splenic artery in SD rats

Objective To establish an animal model of hepatocyte transplantation via splenic artery in SD rats,and to discuss it′s clinic value.Methods Acute liver injury (ALI) was induced by D-gal in 40 SD rats,and they were randomly devided into group A and group B 24 hours later.In group A (n=20),a catheter was inserted into rats′ splenic artery via left common carotid artery,then 1×107 hepatocytes were transplanted into the spleen through the catheter.In group B (n=20),0.5 mL Hank′s solution was infused into the spleen as control through the catheter which inserted into rats′ splenic artery by the same way as group A.At different time points (1,3,6,10,and 15 days),liver function was measured,and secretion of albumin in transplanted hepatocytes was detected by immunofluorescence,and distribution of hepatocyte in spleen was observed by HE stain.Results The success rates of catheterization was 90% in SD rats.In group A,CFDA-SE labeled hepatocytes were found as scattered green fluorescence region in host 1 day after transplantation.Diffused distribution of transplanted hepatocytes were found in host spleen by HE stain 10 days after transplantation.Immunofluorescent staining of albumin demonstrated some positive cells in spleen 15 days after transplantation.Conclusions Hepatocytes can survive in the rat spleen after transplantation through splenic artery.This animal model provides a useful tool for hepatocyte transplantation.