过敏性紫癜患儿Th17、Treg 细胞及IL-17、IL-23 水平的测定

目的:观察过敏性紫癜(Henoch-Schonlein purpura,HSP)患儿急性期及恢复期Th17、Treg 细胞及IL-17、IL-23水平变化,以便更好地认识HSP 的免疫学发病机制,为HSP 的治疗提供帮助。方法:采用流式细胞术(Flow cytometry,FCM)检测HSP 患儿65 例和健康对照组儿童30 例外周血中Th17 细胞和Treg 细胞比例;采用双抗体夹心酶联免疫吸附(ELISA)法测定其血浆中IL-17、IL-23 水平。结果:HSP 急性期组Th17、Th17/ Treg 细胞及IL-17、IL-23 水平高于正常对照组(P<0.05),恢复期组较急性期组降低(P<0.05),但仍高于正常对照组(P<0.05)。HSP 急性期组Treg 水平低于正常对照组(P<0.01),恢复期组较急性期组升高(P<0.01),但是仍低于正常对照组(P<0.01)。单纯型、腹型和其他类型HSP 患儿Th17、Treg、Th17/ Treg 及IL-17、IL-23 水平相同(P>0.05)。HSP 急性期患儿Th17 细胞比例与IL-17 水平呈正相关(r =0.880,P<0.01);HSP 急性期患儿IL-23 水平与Th17 细胞比例、IL-17 水平呈正相关(r =0.838 或r =0.877,P<0.01)。结论:Th17、Treg、Th17/ Treg、IL-17 和IL-23 共同参与HSP 的发病,但在单纯型、腹型和其他类型中的水平无明显差异,值得深入研究。

Detection of Th17,Treg cells and IL-17,IL-23 levels in children with Henoch-Schonlein purpura

Objective:To study the changes of Th17,regulatory T(Treg) cells and IL-17,IL-23 levels at acute phase and recovery phase in children with Henoch-Schonlein purpura(HSP) in order to further understand the immunological pathogenesis and provide help for treating HSP.Methods:The vein blood samples were collected from 65 children with HSP and 30 normal children.The proportion of Th17 cells and regulatory T cells were tested by FCM and concentration of IL-17 and IL-23 in plasma were tested by ELISA.Results:Compared with normal children,the levels of Th17,Th17/ Treg and IL-17,IL-23 were in increase at acute phase in children with HSP(P<0.05).At recovery phase,that were in decrease compared with the acute phase(P<0.05),but still higher than the normal children(P<0.05).Compared with normal children,the level of Treg was in decrease at acute phase in children with HSP (P<0.01).At recovery phase,that was in increase compared with the acute phase(P<0.01),but still lower than the normal children (P<0.01).Among the simplex,abdominal and other type of children with HSP,the levels of Th17,Treg,Th17/ Treg and IL-17,IL-23were same(P>0.05).At acute phase in children with HSP,Th17 cells percentage had positively correlated with IL-17 levels (r =0.880,P<0.01),IL-23 levels had positively correlated with Th17 cells percentage and IL-17 levels (r =0.838 or 0.877,P<0.01).Conclusion:Th17,Treg,Th17/ Treg,IL-17 and IL-23 are involved in the course of the immunological pathogenesis in children with HSP,but the levels of that have no significant difference among simplex,abdominal and other types,further researches need to be done.