利多卡因对脑缺血再灌注损伤大鼠海马组织细胞间黏附分子-1表达的影响

目的观察利多卡因对大鼠脑缺血再灌注损伤后海马组织细胞间黏附分子-1(ICAM-1)基因表达的影响,探讨利多卡因脑保护作用的机制。方法雄性SD大鼠32只,随机分为假手术组(A组)、缺血再灌注组(B组)、利多卡因大剂量组(C组)和利多卡因小剂量组(D组),缺血前10 min腹腔注射。脑缺血10 min再灌注24 h时,断头处死大鼠。用RT-PCR技术检测海马组织ICAM-1的表达,用免疫组织化学、蛋白印记(Western blot)方法检测ICAM-1蛋白表达情况。结果脑缺血再灌注后海马组织ICAM-1 mRNA及蛋白表达水平增高,利多卡因可下调ICAM-1表达,缺血再灌注后,海马区神经元细胞出现明显坏死,利多卡因可减轻海马区神经元损伤。结论利多卡因可能通过抑制ICAM-1表达而对脑缺血再灌注损伤起到一定的保护作用。

Effects of lidocaine on expression of intercellulor adhesion molecule-1 in hippocampus following cerebral ischemia-reperfusion in rats

Objective To investigate the effects of lidocaine on the expression of intercellulor adhesion molecule-1 protein and mRNA in hippocampus following global cerebral ischemia-reperfusion(I/R) in rats. Methods Thirty-two male SD rats were randomly divided equally into 4 groups: group A,sham-operated;group B,I/R;group C,I/R 5 mg lidocaine;group D,I/R 10 mg lidocaine.Cerebral I/R was produced by Longa's method.Reperfusion was done after 10 min cerebral ischemia.The animals were decapitated at the end of 24 h reperfusion and the brains were removed.The levels of ICAM-1 and protein expression in the hippocampus were detected by RT-PCR,Western blot and immunohistochemistry respectively. Results I/R induced ICAM-1 mRNA and protein expression in the hippocampus.Intraperitoneal lidocaine pretreatment significantly inhibited the ICAM-1 expression in hippocampus(B vs C,D,P<0.01). Conclusion Lidocaine can have a protective effect on cerebral ischemia reperfusion injury probably by inhibiting ICAM-1 expression.