斑蝥药材提取工艺与含量测定

目的建立斑蝥药材的提取和含量测定方法。方法以斑蝥素为指标,确定了斑蝥药材的提取方法。采用SE 30气相色谱柱,正十八烷为内标,柱温为175℃保持10 min,10℃.min-1升至210℃,保持15 min,建立了斑蝥素含量测定方法。结果确定最佳提取工艺为:加入15倍量体积分数为75%的乙醇溶液回流提取1h;斑蝥素在0.06~0.60 g.L-1质量浓度内线性关系良好,平均回收率为98.8%,RSD=2.8%(n=9),并对不同产地的斑蝥药材进行了含量测定。结论所建立的含量测定方法适合斑蝥药材中斑蝥素的含量测定。

Extraction process and the determination method of Mylabris phalerata Pallas

Objective To establish the extraction and the content determination method for Mylabris phalerata Pallas.Methods Cantharidin was used as marker component to develop an extraction method of Mylabris phalerata Pallas.A simple and rapid gas chromatographic analysis was also described for the determination of cantharidin in Mylabris phalerata Pallas.It was separated on a SE-30 column with n-ocatadecane as internal standard.Column temperature maintained 10 min at 175 ℃,then raised to 210 ℃ with the flow rate of 10 ℃·min~(-1),and it maintained 15 min at(210 ℃.)Results Extraction process of Mylabris phalerata Pallas was optimized.The linear calibration curve was obtained in the concentration range of 0.06～(0.60 mg·L~(-1).)The mean recovery was 98.9% and RSD was 2.6%(n=9).The method was used for the determination of cantharidin in Mylabris phalerata Pallas from different places.Conclusions The developed method is simple,rapid,accuracy and suitable for quality control of cantharidin in Mylabris phalerata Pallas.