Anordrin对离体培养兔卵泡和黄体的形态学影响

本工作用多杯法培养发情兔的卵巢,观察Anordrin对家兔卵巢卵泡和黄体发育的形态变化。22只发情兔的大卵泡的培养,结果表明,在单纯培养液中加入不同剂量Anordrin处理后,卵泡生长抑制;而培养液中有FSH和LH存在时15微克/毫升或30微克/毫升Anordrin不影响各级卵泡的正常发育,Anordrin增至45微克/毫升则可使成熟卵泡的卵母细胞变性而抑制排卵。18只发情兔的黄体的培养,结果表明,无论培养液中有无FSH和LH存在,不同剂量的Anordrin均可使颗粒黄体细胞显示不同程度的变性和退化,大剂量时还可出现大量泡膜黄体细胞。以上观察表明,卵巢组织经Anordrin处理后,卵泡和黄体显示退化,反映了Anordrin对卵巢有直接的影响。

THE MORPHOLOGICAL EFFECTS OF ANORDRIN ON THE FOLLICLES AND CORPUS LUTEUM OF CULTURED RABBIT OVARIES

The purpose of this study was to observe the morphological changes of the rabbit ovariesafter treatment with various doses of Anordrin in vitro.Ovaries of 22 sexually matured rabbits were cultured in 8 kinds of media.The resultsshowed that follicle growth were retarded in the media containing various doses of Anordrin.The follicles in most ovaries cultured with gonadotrophie hormones plus 15μg/ml or 30μg/ml Anordrin did not effect development of follicles.However,45μg/ml Anordrin plusgonadotrophic hormones induced denaturation of oocytes and suppression of ovulation.Ovaries of 18 sexually matu,re rabbits were cultured in 6 different media.The lutealcells grew normally in media containing FSH and LH or not.Most granulosa luteal cellsshowed denaturation and regression when cultured with various doses of Anordrin or An-ordrin in combination with gonadotrophic hormones,and many thecal luteal cells appearedwhen large doses of Anordrin were added.The above results indicate that Anordrin has a direct effect on the development of rabbitfollicles and corpus luteum.