| 利用cDNA微阵列技术筛选成年期与胚胎期睾丸差异表达基因——钙联接蛋白基因 |
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| 引用本文： | 崔婷,祝辉,王黎熔.利用cDNA微阵列技术筛选成年期与胚胎期睾丸差异表达基因——钙联接蛋白基因[J].中华男科学杂志,2003,9(5):355-357,360. |
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| 作者姓名： | 崔婷 祝辉 王黎熔 |
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| 作者单位： | 1. 南京医科大学第一附属医院检验科,江苏,南京,210029
2. 南京医科大学江苏省生殖医学重点实验室,江苏,南京,210029 |
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| 摘 要： | 目的 :用cDNA微阵列技术克隆和筛选成年期与胚胎期睾丸差异表达基因 ,以进行睾丸发育及精子发生的调控研究。 方法 :构建人睾丸cDNA芯片 ,分别用正常成人及胚胎睾丸的mRNA探针进行杂交 ,对成人和胚胎睾丸中差异表达的基因进行高流量的比较。 结果 :发现 1条成人睾丸高表达、胚胎低表达的基因———钙联接蛋白 (CLGN)基因。 结论 :运用cDNA微阵列方法可筛选到成年期与胚胎期睾丸差异表达基因
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| 关 键 词： | 睾丸 cDNA微阵列 钙联接蛋白基因 |
| 文章编号： | 1009-3591(2003)05-0355-04 |
Molecular Cloning and Identification of Calmegin Gene in Embryo and Adult Testes by cDNA Microarray Hybridization |
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| Ting Cui,Hui Zhu,Li-Rong Wang.Molecular Cloning and Identification of Calmegin Gene in Embryo and Adult Testes by cDNA Microarray Hybridization[J].National Journal of Andrology,2003,9(5):355-357,360. |
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| Authors: | Ting Cui Hui Zhu Li-Rong Wang |
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| Institution: | Department of Clinical Laboratory, First Affiliated Hopital, Nanjing Medical University, Nanjing, Jiangsu 210029, China. |
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| Abstract: | OBJECTIVE: To identify gene differential expression in embryo and adult testes by cDNA microarray techniques. METHODS: cDNA probes of embryo and adult testes were used to hybridize the cDNA microarray of human testis, and the clones of differential hybridization were sequenced and analyzed. RESULTS: The calmegin (CLGN) gene which had been confirmed to be involved in spermatogenesis was found, and it exhibited more than 20-fold difference at expression level between adult and embryo human testes. CONCLUSION: Genes differential expression in adult and embryo human testes can be identified by cDNA microarray hybridization. |
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| Keywords: | Testis cDNA microarray Calmegin gene |
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