系统性红斑狼疮患者Th1/Th2细胞及白介素12、18及其受体mRNA的表达

目的 探讨未经药物治疗的初发系统性红斑狼疮患者Th1/Th2细胞亚群分布,白介素12、白介素18及其受体的基因表达.方法 运用三色荧光标记流式细胞术检测35例初发SLE患者、10例正常人Th1/Th2细胞亚群分布;ABI7700Real-TimePCR法同时检测38例患者和28例正常人IL-12、IL-18及其受体mRNA表达水平.结果 ①初发狼疮患者Th1较正常人明显减低(P<0.05),但Th1/Th2无显着性改变.②与正常人组相比,SLE组患者IL-12、IL-18mRNA及其受体表达较正常人明显降低(P值均<0.05);③面部红斑组患者Th1/Th2、IL-12P35较正常人组降低(P均<0.05);④RNP阳性组患者IL-12P40较正常人组升高,IL-12P35、IL-18较正常人组降低(P均<0.05).结论 SLE是一种以Th1细胞下降,Th2细胞相对占优势的自身免疫性疾病,源于诱导向Th1细胞分化的一系列细胞因子及其受体减少和细胞因子间失衡所致.

Expression of Th1/Th2 and Their Regulatory Cytokines in Systemic Lupus Erythematosus

Objective To analyze Th1/Th2subsets and their regulatory cytokines and cytokine r eceptors in recent onset SLE patients to determi ne the cause of immunological imbala nce in SLE.Methods Th1/Th2 cells were detected quantitatively by flow cytometry in patients with recent-onset SLE(n=35)and the controls(n=10).At the same time,IL-12,IL-12Rβ₂ and IL-18,IL-18R mRNA expression we re tested by ABI7700 real time PCR in SLE patients(n=38/42)and the controls(n=28).Results No difference was found in Th1/Th2 ratio between SLE patients and the controls(P>0.05).However,Th1was decreased significantly in S LE patients than that in the normal controls(P<0.05).The cytokines including IL-12P35,IL-12P40、IL-18and their respective receptors IL-12Rβ₂,IL-18R and IL-12P35/IL-10、IL-18/IL-10 were all significantly decreased in SLE group than those in the controls(P<0.05all).SLE patients with facial erythema presented significantly lower Th1/Th2 ratio and lower IL-12P35 mRNA exp ression than the controls did.Signi ficantly high IL-12P40 expression a s well as low IL-12P35 expression were found in SLE patients with positive RNP.Conclusions SLE is a disease with Th2 predominance due to low Th1 subset and decreased expressions of IL-12 and IL-12Rβ₂,IL-18 and IL-18R mRNA.SLE patients with facial erythema and positive RNP may be the special subtype of SLE,for they have different Th distribution and the particular imbalance in cytokines expression.