| Expression of macrophage inflammatory protein-1α in Kupffer cells following liver ischemia or reperfusion injury in rats |
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| 引用本文： | Ma W,Wang ZR,Shi L,Yuan Y. Expression of macrophage inflammatory protein-1α in Kupffer cells following liver ischemia or reperfusion injury in rats[J]. World journal of gastroenterology : WJG, 2006, 12(24): 3854-3858. DOI: 10.3748/wjg.v12.i24.3854 |
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| 作者姓名： | Ma W Wang ZR Shi L Yuan Y |
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| 作者单位： | [1]Department of Hepatobiliary.Surgery, First Hospital of Xi＇an Jiaotong University, Xi＇an 710061, Shaanxi Province, China [2]Department of Pharmacy, First Hospital of Xi＇anJiaotong University, Xi＇an 710061, Shaanxi Province, China |
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| 摘 要： | ![]()
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| 关 键 词： | 巨噬细胞炎性蛋白-1α 肝疾病 缺血再灌注损伤 病理机制 |
| 收稿时间： | 2006-01-05 |
Expression of macrophage inflammatory protein-1alpha in Kupffer cells following liver ischemia or reperfusion injury in rats |
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| Ma Wei,Wang Zuo-Ren,Shi Lei,Yuan Yue. Expression of macrophage inflammatory protein-1alpha in Kupffer cells following liver ischemia or reperfusion injury in rats[J]. World journal of gastroenterology : WJG, 2006, 12(24): 3854-3858. DOI: 10.3748/wjg.v12.i24.3854 |
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| Authors: | Ma Wei Wang Zuo-Ren Shi Lei Yuan Yue |
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| Affiliation: | Department of Hepatobiliary Surgery, First Hospital of Xi'an Jiaotong University, Shaanxi Province, China. wowotoumario@yahoo.com.cn |
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| Abstract: | ![]()
AIM: To explore the expression of macrophage inflammatory protein-1alpha (MIP-1alpha) in Kupffer cells (KCs) following liver ischemia/reperfusion injury IRI in rats. METHODS: Forty male SD rats were divided randomly into five groups. A model of partial warm ischemia/reperfusion injury in the rat liver was established. KCs were isolated and incubated one hour, six hours, 12 h, and 24 h after the reperfusion. Tumor necrosis factor alpha (TNF-alpha) and interleukin-1beta (IL-1beta) in the supernatants were measured by ELISA. MIP-1alpha in KCs was detected by immunocytochemical and RT-PCR. RESULTS: No or few MIP-1alpha protein and mRNA were expressed in the KCs of the control group. Its expression in the IRI group had a significant increase after the reperfusion (P < 0.05), which was contrary to the control group. CONCLUSION: The active behavior of the MIP-1alpha gene in KCs following liver ischemia/reperfusion injury is assumed to be one of the major causes for the hepatic ischemia/reperfusion injury. |
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| Keywords: | Liver Ischemia/reperfusion Kupffer cell Macrophage inflammatory protein-1α |
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