| 尿多酸肽(CDA-2)诱导多发性骨髓瘤细胞RPMI8226凋亡的实验研究 |
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| 引用本文： | 叶宝东,沈一平,周郁鸿,邵科钉,俞庆宏.尿多酸肽(CDA-2)诱导多发性骨髓瘤细胞RPMI8226凋亡的实验研究[J].浙江中医药大学学报,2010,34(5). |
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| 作者姓名： | 叶宝东 沈一平 周郁鸿 邵科钉 俞庆宏 |
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| 作者单位： | 浙江中医药大学附属第一医院,杭州,310006 |
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| 摘 要： | 目的]观察尿多酸肽(CDA-2)对多发性骨髓瘤细胞RPMI8226抑制增殖和凋亡作用。方法]应用MTT法,检测CDA-2对多发性骨髓瘤细胞株RPMI8226增殖的影响。通过Hochest33258染色、流式细胞仪(AnnexinV和PI双染色)检测细胞凋亡。结果]CDA-2明显抑制RPM18226细胞的增殖。CDA-2作用RPM18226细胞增殖24h的半数抑制浓度(IC50)分别为1.64mg/g。采用Hochest33258染色,荧光显微镜下观察CDA-2以2mg/g及4mg/g终浓度作用于RPM1822624h后细胞形态改变,可见明显的凋亡小体出现。AnnexinV-PI双标记法流式细胞仪分析结果显示,RPMI8226细胞2mg/gCDA-2作用6、12、24h后,早期凋亡细胞百分数分别为5.43%、12.02%、29.07%,明显高于空白对照组4.88%。结论]CDA-2对MM细胞株RP-MI8226有生长抑制作用,可能是通过诱导其发生凋亡而起作用。
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| 关 键 词： | 多发性骨髓瘤 尿多酸肽 细胞凋亡 |
The Apoptosis-inducing Effect of Uroacitides(CDA-2)on Human Multiple Myeloma RPMI8226 Cells |
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| Institution: | Ye Baodong; Shen Yiping; Zhou Yuhong; et al First Hospital Affiliated to Zhejiang Chinese Medical University; Hangzhou(310006); |
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| Abstract: | Objective]To investigate the proliferation inhibition and apoptosis-inducing effect of uroacitides(CDA-2)on human multiple myeloma RPMI8226 cells.Methods]MTT assay was undertaken to observe the growth inhibition of RPMI8226 cells exposed to CDA-2.The apoptosis of RPMI8226 cells were detected by fluorescence staining with hochest33258,flow cytometry(Annexin V and PI staining).Results]MTT analysis indicated that the proliferation of RPMI8226 cells were inhibited significantly with CDA-2 treatment for 24h.T... |
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| Keywords: | multiple myeloma CDA-2 cell apoptosis |
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