| 药用紫锥菊叶片离体培养技术研究 |
| |
| 引用本文： | 李标,唐坤,刘毅,王伯初.药用紫锥菊叶片离体培养技术研究[J].时珍国医国药,2006,17(3):344-345. |
| |
| 作者姓名： | 李标 唐坤 刘毅 王伯初 |
| |
| 作者单位： | 1. 重庆邮电学院生物信息学院,重庆,400065;重庆大学生物工程学院,重庆,400044
2. 重庆邮电学院生物信息学院,重庆,400065 |
| |
| 基金项目： | 重庆市科委自然科学基金项目(No.CSTC2005BB1094)，重庆邮电学院青年教师基金资助项目(No.A2004-23) |
| |
| 摘 要： | 目的研究药用紫锥菊叶片离体培养技术,建立最佳培养条件。方法在紫锥菊的种子苗上采取叶片,接种到附加不同生长物质的培养基中,诱导其成为完整植株。结果对于药用紫锥菊的叶片,适宜在MS IBA 2 mg/L NAA 1 mg/L培养基上分化成苗;在MS IBA 0.2 mg/L BA 0.7 mg/L GA 0.5 mg/L培养基上增殖,增殖率可达5~6;无根苗在1/2MS IBA 0.5 mg/L NAA 0.1 mg/L培养基上迅速长根,生根率很高。结论采用药用紫锥菊的叶片作为外植体,利用组织培养的方法能够实现种苗的工厂化快速繁殖。
|
| 关 键 词： | 紫锥菊 叶片 离体培养 |
| 文章编号： | 1008-0805(2006)03-0344-02 |
| 修稿时间： | 2005年7月5日 |
A Study on Leaves Vitro Culture Technology of Medicine Echiancea purpurea |
| |
| LI Biao,TANG Kun,LIU Yi,WANG Bo-chu.A Study on Leaves Vitro Culture Technology of Medicine Echiancea purpurea[J].Lishizhen Medicine and Materia Medica Research,2006,17(3):344-345. |
| |
| Authors: | LI Biao TANG Kun LIU Yi WANG Bo-chu |
| |
| Abstract: | ObjectiveTo set up the optimums for leaves vitro culture of medicine Echiancea purpurea.MethodsLeaves from seedlings were cut and cultured into the different media containing different promoting growth substance,and induced to form the whole plantlet.ResultsAs to leaves of medicine E.purpurea,adapting to grow the shoots by differentiation on the MS IBA 2 mg/L NAA 1 mg/L culture medium;On another culture medium,the MS IBA 0.2 mg/L BA 0.7 mg/L GA 0.5 mg/L medium,they can propagate quickly and the multiplication rate will reach 5~6.Meanwhile,the differential shoots will grow roots promptly on the 1/2 MS IBA 0.5 mg/L NAA 0.1 mg/L medium.As anticipated,the radicant rate can reach a high level.ConclusionActing leaves of medicine E.purpurea as explants,can carry out the rapid propagation by tissue culture. |
| |
| Keywords: | E purpurea Leaf Vitro culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
