|
|||||
|
|
Quantification of Human Immunodeficiency Virus Type 1 RNA Levels in Plasma by Using Small-Volume-Format Branched-DNA Assays |
|
| Authors: | Torange Yeghiazarian Yuqi Zhao Stanley E. Read William Kabat Xiaoyi Li Sarah J. Hamren Patrick J. Sheridan Judith C. Wilber David N. Chernoff Ram Yogev |
| Affiliation: | Chiron Diagnostics, Emeryville, California1.; Section of Pediatric and Maternal HIV-1 Infections, Division of Infectious Diseases, Children’s Memorial Hospital,2. and Department of Pediatrics3. and Department of Microbiology and Immunology,4. Northwestern University Medical School, Chicago, Illinois; and Division of Infectious Diseases, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada5. |
| Abstract: | We have developed small-volume (50 or 250 μl)-format branched-DNA assays for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is anticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes. |
| Keywords: | |