二色桂木正丁醇提取物的抗抑郁活性研究

目的:探讨二色桂木提取物的抗抑郁活性及其作用机制。方法:采用KM种小鼠(雄性)悬尾、强迫游泳和利血平诱导抑郁症模型,以ig连续给药10 d(空白组等容生理盐水;氟西汀组50 mg·kg-1;二色桂木粗提物及其石油醚、乙酸乙酯、正丁醇部位及水部位各给药组200 mg·kg-1),研究二色桂木醇提物及其不同极性部位的抗抑郁活性;采用荧光分光光度法测定海马及纹状体5-羟色胺(5-HT)。结果:二色桂木醇提物及石油醚部位、乙酸乙酯部位、正丁醇部位和水部位均可不同程度地缩短小鼠游泳和悬尾的不动时间,其中正丁醇部位的作用最显著(P<0.05或P<0.01),并能显著拮抗利血平引起的体温下降和眼睑下垂等生理变化(P<0.05或P<0.01),显著提高5-HT在海马、纹状体中的含量(P<0.05或P<0.01)。结论:二色桂木醇提物具有抗抑郁作用,其活性成分主要分布在正丁醇部位。

Antidepressant Activity of N-butanol Extracts from Artocarpus styracifolius Pierre

Objective: To study the antidepressant activity and mechanism of extracts from Artocarpus styracifolius Pierre (AS). Method: Antidepressant activity was tested by tail suspension test, force swimming test and reserpine model of different polar extracts from AS, and serotonin (5-HT) content was determined by fluorescence spectrophotometry. AS was given by intragastric administration for 10 days (fluoxetine group 50 mg· kg^-1; each AS group 200 mg· kg^-1). Result: The different polar extracts of AS could shorten immobility time in tail suspension test and force swimming test on mice(P<0.05 or P<0.01). Action of n-butanol extract of AS could have an antagonistic action on reserpine caused drop of body temperature and blepharoptosis and improved 5-HT content in hippocampal convolution and corpus striatum on mice(P<0.05 or P<0.01). Conclusion: Extracts of AS had antidepressant activity, and activity part may be n-butanol extract.