北京地区妇女宫颈人乳头瘤病毒感染及亚型分布情况分析

目的探讨北京地区妇女宫颈人乳头瘤病毒(HPV)感染及其亚型分布情况。方法采用聚合酶链反应(PCR)体外扩增和脱氧核糖核酸(DNA)反向点杂交相结合的DNA芯片技术,对920例宫颈脱落细胞标本进行HPV基因分型检测。结果 920例标本共检出HPV感染508例,总感染率为55.22%。23种亚型共检出21种,高危亚型MM4及低危亚型44未检测出;感染率居前五位的基因亚型是HPV-16 238例(24.74%),HPV-11 100例(10.4%),HPV-6 95例(9.88%),HPV-58 84例(8.73%)和HPV-33 75例(7.8%)。单一基因型感染285例,占总感染数的56.1%;多重感染223例,占总感染数的43.9%。<20、20～29、30～39、40～49和≥50岁五个不同年龄组阳性感染构成比分别为1.97%、25.78%、37.60%、23.43%和11.22%。结论北京地区妇女宫颈HPV感染率较高,HPV感染高发年龄段为20～49岁性活跃阶段女性,HPV基因分型检测技术对宫颈癌预防有积极作用。

Human papillomavirus infection in married women and its subtype distribution in Beijing

Objective To study the human papillomavirus(HPV) infection in married females and its subtype distribution.Methods PCR and gene chip technology were used to test 920 cervical cell samples,and 23 HPV genotypes were detected.Results Among 508 positive samples,21 subtypes of 23 known subtypes were detected.The low risk subtype HPV-44 and high risk subtype HPV-MM4 had not been detected.Altogether 508(55.22%)women were HPV positive among 920 patients.Of 508 HPV positive cases,238(24.74%) were HPV-16,100(10.4%) were HPV-11,95(9.88%) were HPV-6,84(8.73%) were HPV-58,and 75(7.8%) were HPV-33.285 cases were single HPV type infection,223 cases were double and multiple HPV types infection.HPV positive rates in different age groups(<20,20~29,30~39,40~49 and ≥50) were 1.97%,25.78%,37.60%,23.43% and 11.22%,respectively.Conclusion HPV infection in woman in Beijing was severe.The distinction of HPV genotypes play an important role in prevention and management of cervical cancer.