| HPLC法测定化瘀祛斑片芍药苷的含量 |
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| 引用本文： | 吴健,马丽萍,王如伟,何林旺,程志宏. HPLC法测定化瘀祛斑片芍药苷的含量[J]. 中国现代药物应用, 2009, 3(14): 27-28 |
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| 作者姓名： | 吴健 马丽萍 王如伟 何林旺 程志宏 |
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| 作者单位： | 1. 浙江康恩贝药品研究开发有限公司,310052
2. 浙江松寿堂中药有限公司 |
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| 摘 要： | 目的建立HPLC法测定化瘀祛斑片中芍药苷含量测定的方法。方法采用HPLC法测定片中芍药苷的含量。采用Diamonsil C18色谱柱（250mm×4.6mm，5μm），以乙腈一水-磷酸（20：80：0.1）为流动相，流速为1.0ml／min，检测波长230nm，进样量为10μl。结果芍药苷在0.3964—3.5676mg／mL与峰面积呈良好的线性关系，r=0.9999，平均加样回收率为99.2％，RSD为1.51％（n=6）。结论本方法简便，准确，灵敏度高，重现性好，可用于化瘀祛斑片中芍药苷的HPLC含量测定。
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| 关 键 词： | 化瘀祛斑片 芍药苷 含量测定 高效液相色谱法 |
Determination of Paeoniflorin in Huayuquban Tablets With HPLC Method |
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| Affiliation: | WU Jian, MA Li-ping, WANG Ru-wei, et al.( Drug Research and Development a Limited Company of Zhejiang, Kangenbei, Hangzhou, 310052, China) |
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| Abstract: | Objective to establish HPLC method for determination of Paeoniflorin in Huayuquban Tablets. Methods The chromatographie conditions consisted of Diamonsil C18 colunm（250mm×4.6mm,5μm） with the mobile phase of aeetonitrile-water-chemical reagent phosphoric acid （20 ： 80 ： 0.1） at the flow rate of 1.0ml/min, the deter-ruination wavelength was 230 nm and sample size was 10μl. Results Paeoniflorin showed a good linearity at the range of 0.3964—3.5676 mg/mL , r = 0.9999, average recovery was 99.2% , RSD% = 1.51% （ n = 6）. Conclusion The method is simple, accurate, high sensitive and good reproducibility, ean be used as the method for determination of Paeoniflorin in Huayuquban Tablets. |
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| Keywords: | Huayuquban Tablets Paeoniflorin Content determination HPLC method |
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