解酒保肝口服液对小鼠酒精中毒的影响

目的：观察解酒保肝口服液对小鼠醉酒实验,血清乙醇浓度和肝、胃组织乙醇脱氢酶活性的影响。方法：将生理盐水和将葛根,甘草等中药用水煎煮制成解酒保肝口服液灌服于小鼠后30min,灌服白酒,记录小鼠翻正反射消失（醉酒）至恢复（醒酒）所需时间（min),及24h内小鼠的死亡只数,另以相同操作连续6d后眼眶取血并处死动物,立即取出肝脏和胃,分别用生化比色法测定血肖乙醇浓度和肝、胃组织乙醇脱氢酶活性。结果：在醉酒实验中,。与对照组相比服用解酒保肝口服液组小鼠从饮酒到翻正反射消失（醉酒）的时间明显延长（P＜0．01）,醒酒时间明显缩短,且小鼠的死亡率明显降低（P＜0．05）,血清乙醇含量明显降低,肝脏ADH高于对照组, 结论：解酒保肝口服液具有解酒作用。

Experimental study on the effects of Jiejiubaogankoufuye on ethanol intoxication in mice

OBJECTIVE To investigate the effects of Jiejiubaogankoufuye on experimental mice drunk, the concentration of ethanol in serum and the activity of alcohol dehydrogenase in liver and stomach tissue in mice with ethanol intoxication.METHODS All mice were p.o white spirit after 30 min of p.o saline or " Jiejiubaogankoufuye " made mainly with lobed kudzurine root and liquorice root, and the durations from the disappear of righting reflex to recovery in mice were recorded and the number of dead mice in 24 hours were countered; With the same procedure for six days,the mice were killed by withdrawaling blood from orbit, and resected liver and stomach at once, the concentration of ethanol in serum and the activities of alcohol dehydrogenase in liver and stomach were measured by biochemical chromatometry respectively.RESULTS In drunk test, " Jiejiubaogankoufuye "significantly postponed the time from p.o white spirit to the disappear of righting reflex( P < 0.01 ),shortened the duration of drunk and decreased the death rate of mice( P < 0.05 ) comparing with control group;The serum concentration of ethanol was obviously lower and the activity of alcohol dehydrogenase in liver was higher than control group.CONCLUSIONS " Jiejiubaogankoufuye " might alleviate ethanol intoxication.