过表达GATA-4的小鼠骨髓间充质干细胞改善小鼠心肌梗死后的心功能

目的探讨过表达GATA-4的小鼠骨髓间充质干细胞(BMSCs)对小鼠心肌梗死后心功能的影响。方法通过慢病毒载体携带GATA-4感染小鼠BMSCs,构建过表达GATA-4的小鼠BMSCs。将过表达GATA-4-BMSCs,空载体-BMSCs(NC-BMSCs)及BMSCs用于低氧培养诱导细胞凋亡。48h后,用流式细胞计量术检测细胞凋亡率;用Westernblot检测caspase-3、caspase-9和β-actin及细胞色素C的表达。给心肌梗死模型小鼠尾静脉注射上述细胞,用心脏彩超检测48h心功能;用TUNEL技术检测心肌梗死局部凋亡细胞数量。结果过表达GATA-4-BMSCs组与BMSCs组、NC-BMSCs组比较具有较强的抗凋亡能力(P<0.05)。GATA-4-BMSCs组的caspase-8和cytochromeC表达量最低(P<0.05)。过表达GATA-4-BMSCs对射血分数及左室短轴缩短率改善幅度最大(P<0.05)。48h后,过表达GATA-4-BMSCs组心肌梗死局部凋亡细胞数量减少(P<0.05)。结论过表达GATA-4-BMSCs可以通过增强BMSCs抗凋亡,有效改善小鼠心肌梗死后的心功能。

GATA-4-over-expressed bone marrow mesenchymal stem cells improve cardiac function after myocardial infarction in mice

Objective To explore the effect of GATA-4-over-expressed bone marrow mesenchymal stem cells(BMSCs)in mice on cardiac function after myocardial infarction.Methods GATA-4-over-expressed BMSCs were constructed by transfecting BMSCs with lentiviral vector carrying GATA-4.Subsequently,GATA-4-over-expressed BMSCs,GATA-4-free-vector-BMSCs,and BMSCs were subjected to hypoxia culture to induce apoptosis.After 48 h,cell apoptosis rate was determined by flow cytometry,whereas caspase-3,caspase-9,β-actin,and cytochrome C were measured by Western blot.The mouse model of myocardial infarction was used and established for 48 h.Above group of cells were injected into the tail veins.The cardiac function was examined by cardiac color Doppler ultrasonography.Moreover,the number of local apoptotic cells in myocardial infarcted mice was detected by TUNEL technique.Results GATA-4-over-expressed-BMSCs group had stronger anti-apoptotic ability(P<0.05).The minimal expression of caspase-8 and cytochrome C occurred in GATA-4-BMSCs group(P<0.05).Ejection fraction and contraction ratio also exhibited maximal degree improvement in GATA-4-over-expressed-BMSCs group(P<0.05).Number of local apoptotic cells in myocardial infarcted mice was lower in GATA-4-over-expressed-BMSCs group(P<0.05).Conclusions GATA-4-over-expressed-BMSCs can enhance the anti-apoptotic function of BMSCs to improve the cardiac function effectively after myocardial infarction.