ACETALDEHYDE ADDUCTS WITH HAEMOGLOBIN: DETERMINATION OF ACETALDEHYDE RELEASED FROM HAEMOGLOBIN BY ACID HYDROLYSIS |
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Authors: | LUCAS, D. MENEZ, J. F. BODENEZ, P. BACCINO, E. BARDOU, L. G. FLOCH, H. H. |
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Affiliation: | Laboratoire de Biochimie, UA CNRS 598, Faculté de Médecine 22 avenue Camille Desmoulins, 29285 Brest Cédex, France |
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Abstract: | Acetaldehyde, the first metabolite of ethanol, reacts with haemoglobinin vitro to produce acetaldehydehaemoglobin adducts.Some clinical studies on the minor haemoglobins have suggestedthat these adducts may be formed in people abusing alcohol.Under hydrolysis of haemoglobin, with oxalic acid at 100°Cin sealed vials, some acetaldehyde was released and then specificallydetermined by HPLC. The kinetics of hydrolysis were studiedusing haemoglobin previously labelled with 14[C] acetaldehyde.The maximum liberation of 14[C] acetaldehyde was obtained after3 hr 30 min hydrolysis and this time factor was then utilizedin the analysis of alcoholic and control haemoglobin. Thus,we have confirmed the formation of acetaldehyde haemoglobinadducts in vivo. It must be noted that the released acetaldehydecorresponds only to an index of the stable adducts. The levelswere higher in alcoholics than in controls (1.417±0.171and 1.295±0.139 nmol/mg Hb, respectively, P<0.001).In conclusion, this marker is not a convenient tool for themonitoring of alcohol exposure levels because of the low differencesbetween alcoholic and control haemoglobins. |
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