扶正化瘀方对大鼠肝大部切除后肝再生的影响

目的 探讨扶正化瘀方对大鼠肝大部切除(PH)后肝再生的影响.方法 将28只正常雄性Wistar大鼠随机分为4组,分别为假手术组(对照组)、PH模型(PH)组、PH+扶正化瘀组和PH+促肝细胞生长素(pHGF)组,每组7只.扶正化瘀组和PH +pHGF组于实验前3天至实验结束,分别给予扶正化瘀方药液灌胃1 ml/l00 g和pHGF腹腔注射1 ml/100 g,每日1次.术后24 h心脏采血后处死大鼠,计算各组肝再生速度,血清标本检测ALT、AST、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)水平,免疫组织化学染色和荧光定量PCR检测肝组织cyclinD1的表达.结果 PH组、PH +pHGF组和PH+扶正化瘀组ALT、AST水平明显升高,白蛋白水平明显下降,与对照组比较差异均有统计学意义(均P ＜0.01).与对照组比较,PH组、PH +pHGF组和PH+扶正化瘀组血清TNF-α和IL-6水平均明显升高(均P＜0.01).与对照组比较,PH组、PH+扶正化瘀组和PH +pHGF组大鼠有丝分裂指数、肝组织cyclin D1 mRNA和肝细胞核cyclin D1阳性率均明显升高(均P＜0.01)；PH+ PHGF组和PH+扶正化瘀组肝再生速度明显高于PH组[(22±4)％、(20±2)％比(13±1)％,P＜0.01].与对照组比较,PH组、PH +pHGF组和PH+扶正化瘀组有丝分裂指数、肝组织cyclinD1 mRNA和肝细胞核cyclin D1阳性率均明显升高[有丝分裂指数:(25.90±0.95),(29.70±0.50),(30.30±0.96)％比(0.95±0.19)％；肝组织cyclin D1 mRNA:2.18±0.37,2.73±0.29,2.93±0.18比1.03±0.09；肝细胞核cyclin D1阳性率:(27.20±0.36)％,(28.30±0.16)％,(29.30±0.21)％比(0.95±0.19)％](P＜0.01或P＜0.05),而PH+扶正化瘀组和PH +pHGF组间差异无统计学意义(P＞0.05).结论 扶正化瘀方可通过增强cyclinD1表达而促进大鼠肝大部切除术后的肝再生.

Effect of Fuzheng huayu decoction on liver regeneration after partial hepatectomy in rats

Objective To investigate the effect and possible mechanism of Fuzheng huayu decoction on liver regeneration in rats subsequent to partial hepatectomy.Methods Male Wistar rats were randomized into 4 groups：sham-operation rats （control group）,partial hepatectomy rats （PH group）,partial hepatectomy plus Fuzheng huayu decoction rats （PH ＋ Fuzheng group）,partial hepatectomy plus pHGF rats （PH ＋ pHGF group）.24 h after hepatectomy,rats were sacrificed; blood and organs were removed.Serum aspartate aminotransferase,alanine aminotransferase,tumor necrosis factor α （TNF）-α and intedeukin-6 （IL-6） levels were determined.The liver regeneration rate of the rats and the mitotic index were calculated.The gene and protein expression of cyclinD1 in liver tissue were evaluated by real-time PCR and immunohistochemical staining.Results Alanine aminotransferase （ALT） and aspartate aminotransferase （AST） levels were significantly increased and albumin levels were significantly decreased in PH group,PH ＋ pHGF group and PH ＋ Fuzheng group compared with the control group; the differences were statistically significant （P 〈 0.01）.Compared with the control group,serum TNF-α and IL-6 levels in the PH group,PH ＋ the of pHGF group and PH ＋ Fuzheng group were significantly elevated （both P 〈 0.01）.Compared with control group,the mitotic index,the gene and protein expression of cyclinD1 increased significantly in PH group,PH ＋ Fuzheng group and PH ＋ pHGF group at the 24 hour after partial hepatectomy.The mitotic index,cyclinD1 and liver regeneration rate of the rats increased significantly in PH ＋ Fuzheng group and PH ＋ pHGF group than that in PH group.Conclusion Fuzheng huayu decoction can improve liver regeneration by a mechanism related to the up-regulation of cyclinD1 gene expression.