| 人胚胎干细胞饲养层的制备及生物学活性 |
| |
| 引用本文： | 胡嘉波,麻全慧,胡三强,周中卫,王晓慧,钱晖,许文荣.人胚胎干细胞饲养层的制备及生物学活性[J].中国组织工程研究与临床康复,2011,15(23):4233-4236. |
| |
| 作者姓名： | 胡嘉波 麻全慧 胡三强 周中卫 王晓慧 钱晖 许文荣 |
| |
| 作者单位： | 江苏大学基础医学与医学技术学院,江苏省镇江市,212001 |
| |
| 基金项目： | 江苏大学高级人才专项资助，国家自然科学基金资助项目 |
| |
| 摘 要： | 背景:建立一种既可以大量制备,又能保存并保持较高活性的饲养层是胚胎干细胞培养研究不可缺少的环节。目的:体外分离培养、冻存复苏ICR小鼠胚胎成纤维细胞,观察其生物学特性。方法:取ICR小鼠13.5d胚胎,用胰蛋白酶分步消化法分离培养小鼠成纤维细胞,对冻存复苏后的小鼠胚胎成纤维细胞形态、生长曲线、贴壁率、细胞化学染色及支持人胚胎干细胞生长特性等进行观察。结果与结论:复苏后的小鼠胚胎成纤维细胞在体外传代30min时80%以上细胞贴壁,生长曲线显示细胞增殖活跃,细胞化学染色AKP、PAS、POX阴性,能长期支持人胚胎干细胞传代生长。提示此方法所获得的小鼠胚胎成纤维细胞复苏后有较高的生物学活性,可为人胚胎干细胞扩增提供稳定、优质的饲养层细胞。
|
| 关 键 词： | 小鼠胚胎成纤维细胞 ICR小鼠 人胚胎干细胞 冻存 复苏 |
Preparation and biological characteristics of feeder cells for human embryonic stem cells |
| |
| Hu Jia-bo,Ma Quan-hui,Hu San-qiang,Zhou Zhong-wei,Wang Xiao-hui,Qian Hui,Xu Wen-rong.Preparation and biological characteristics of feeder cells for human embryonic stem cells[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(23):4233-4236. |
| |
| Authors: | Hu Jia-bo Ma Quan-hui Hu San-qiang Zhou Zhong-wei Wang Xiao-hui Qian Hui Xu Wen-rong |
| |
| Institution: | Hu Jia-bo,Ma Quan-hui,Hu San-qiang,Zhou Zhong-wei,Wang Xiao-hui,Qian Hui,Xu Wen-rong School of Medical Science and Laboratory Medicine,Jiangsu University,Zhenjiang 212001,Jiangsu Province,China |
| |
| Abstract: | BACKGROUND:It is important to establish and save a large number of high-activity feeder cells for the culture of human embryonic stem cells(hESCs) . OBJECTIVE:To separate,culture,freeze and thaw embryonic fibroblasts of ICR mice in vitro,and to explore the biological characteristics of mouse embryonic fibroblast. METHODS:ICR mouse fetuses of 13.5 days were chosen to isolate mouse embryonic fibroblasts by trypsin stepwise. We observed the cell morphology,growth curves,cells adhesive rate,and cytochemistry st... |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
