| 基因Hath1表达对结肠癌细胞的抑制作用 |
| |
| 引用本文： | 赵丽丽,刘忠,张渝洁. 基因Hath1表达对结肠癌细胞的抑制作用[J]. 中国医药生物技术, 2011, 6(3): 204-208. DOI: 10.3969/cmba.j.issn.1673-713X.2011.03.007 |
| |
| 作者姓名： | 赵丽丽 刘忠 张渝洁 |
| |
| 作者单位： | 1. 山东鲁南制药集团有限公司,临沂,276006
2. 临沂大学生命科学学院,临沂,276005 |
| |
| 摘 要： | 目的研究Hath1基因对结肠癌细胞体外增殖的抑制作用。方法用trizol试剂提取肿瘤细胞总RNA,定量逆转录PCR法检测Hath1在结肠癌细胞和正常组织细胞中的表达;MTS和流式细胞仪法分别检测Hath1在结肠癌细胞中的增殖率和凋亡百分率;构建IEC-6稳定表达Hath1siRNA细胞系,检测Hath1siRNA对IEC-6细胞生长的影响。结果在正常人的小肠与结肠组织及大鼠小肠上皮细胞IEC-6中有着比较高水平的Hath1的表达,而在4株结肠癌细胞中Hath1的表达都很低。在表达Hath1的4株结肠癌细胞SW480、HT29、LS174T、SW620中,其细胞增殖率分别被抑制了38.5%、23.4%、55%、35.6%。在LS174T细胞中Hath1不能有效地诱导肿瘤细胞发生凋亡,但是可以显著地提高肿瘤细胞对化疗药物的敏感性。siRNA干扰试验进一步证明了Hath1起到肿瘤抑制基因的作用。结论 Hath1不能有效地诱导肿瘤细胞发生凋亡,但是可以显著地提高肿瘤细胞对化疗药物的敏感性。
|
| 关 键 词： | 转录因子 细胞系,肿瘤 基因,肿瘤抑制 RNA,小分子干扰 |
Hath1 gene inhibits colorectal cancer cell growth |
| |
| ZHAO Li-li,LIU Zhong,ZHANG Yu-jie. Hath1 gene inhibits colorectal cancer cell growth[J]. Chinese Medicinal Biotechnology, 2011, 6(3): 204-208. DOI: 10.3969/cmba.j.issn.1673-713X.2011.03.007 |
| |
| Authors: | ZHAO Li-li LIU Zhong ZHANG Yu-jie |
| |
| Affiliation: | Lunan Pharmaceutical Group Corporation,Linyi 276006,China (ZHAO Li-li,LIU Zhong),College of Life Science,Linyi University,Linyi 276005,China (ZHANG Yu-jie) |
| |
| Abstract: | Objective The inhibitory effect of Hath1 gene on the growth of colorectal cancer cell in vitro.Methods The total RNA of tumor cells was extracted by trizol.The expression level of Hath1 in colorectal cancer cells and normal tissue cells were detected by real time PCR.MTS attest and flow cytometry were used to assess the proliferation and cell apoptosis of colorectal cancer cells.IEC-6 cell line that can stably express Hath1 siRNA was constructed and the effect of Hath1 siRNA on the growth of IEC-6 cell was ... |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
