URINARY MONOHYDROXY BILE ACIDS IN YOUNG INFANTS WITH OBSTRUCTIVE JAUNDICE

ABSTRACT. Using an aluminum oxide column, we fractionated and quantitatively determined urinary monohydroxy bile acids in young infants. For comparison purposes, monohydroxy bile acids were also measured in urine from older children and adults with obstructive jaundice. Lithocholic acid was not found in any specimens of the young infants examined, while 3β-hydroxy-5-cholenoic acid was detected in all. In the biliary atresia group, 3β-hydroxy-5-cholenoic acid excreted was 0.45 ± 0.28 μmol per day ( n = 7), and in the neonatal hepatitis group, 0.48 ± 0.44 μmol per day ( n = 9). The mean rate of 3β-hydroxy-5-cholenoic acid to total urinary bile acids in the biliary atresia group was 2.1%, and 1.3% in the neonatal hepatitis group. In the older children and adults with obstructive jaundice ( n =6), 3β-hydroxy-5-cholenoic acid was excreted at a mean rate of 3.9% of total urinary bile acids, ranging from 0.63 to 14.81 mol per day. The excretion rate of 3β-hydroxy-5-cholenoic acid was related to that of chenodeoxycholic acid ( p <0.05) in infants, while it was related to that of both chenodeoxycholic acid ( p <0.01) and cholic acid ( p <0.05) in older children and adults.