小鼠胚胎心流出道的形态发生与呼吸内胚层发育耦联

目的 探讨小鼠胚胎心发育过程中前肠呼吸内胚层与第一生心区和第二生心区及胚胎心流出道的形态发生关系。方法 胚龄7.5～13d小鼠胚胎心各3个，连续石蜡切片，用抗胰岛素增强子结合蛋白1（ISL-1）、抗心肌肌球蛋白重链（MHC）、抗α-平滑肌肌动蛋白（α-SMA）、抗Nkx2.5和抗增殖细胞核抗原（PCNA）抗体进行免疫组织化学或免疫荧光染色。结果 胚胎发育7.5d，ISL-1在生心板心肌前体细胞表达并与发育中的第二生心区ISL-1阳性细胞连续。胚胎发育第10～13天， 可见ISL-1强阳性的前肠腹侧呼吸内胚层细胞增生，排列不规则，

Coupling of heart outflow tract morphogenesis with development of pulmonary endoderm in mouse embryo

Objective To explore the morphological relationship of the pulmonary endoderm with the primary heart field (PHF), the second heart field (SHF) and the heart outflow tract during early development of mouse embryonic heart. Methods Serial sections of three mouse embryos each day from embryonic day(ED) 7.5 to embryonic day(ED) 13 were stained immunohistochemically or immunofluorescently with antibodies against insulin gene enhancer binding protein (Islet-1，ISL-1), myosin heavy chain (MHC),α-smooth muscle actin (α-SMA), Nkx2.5 and proliferating cell nuclear antigen (PCNA). Results At ED7.5, ISL-1 positive cells in the cardiogenic plate were continuous with that of the developing SHF. From ED10 to ED13, ISL-1 positive pulmonary endoderm cells showed strong proliferation, irregular arrangement, loss the polarity with apparent intercellular space and changed to mesenchyme-like cells without obvious border with surrounding ISL-1 positive mesenchyme. Local proliferation of endoderm cells adjacent closely to the dorsal wall of aortic sac resulted in the formation of solid endoderm cord that extended towards aortic sac. Ventral to the foregut, ISL-1 positive mesenchyme cells surrounding the pulmonary endoderm and endoderm cord formed a distinctive cone-shaped structure. Conclusion PHF and SHF is a continuum and ISL-1 is an early marker for cardiac precursor cells in both structures. Pulmonary endoderm cells and the endoderm cord might play roles in inducing proliferation of ISL-1 positive cells or might transform to mesenchymal cells via epithelium-mesenchyme transition to maintain certain cell density of S