替拿素在子宫颈鳞状细胞癌组织表达及临床意义初步研究

目的探讨子宫颈鳞状细胞癌（宫颈鳞癌，SCC）组织替拿素表达及其临床意义。方法采用免疫组织化学SP法检测28例正常宫颈上皮（NCE）、36例宫颈上皮内瘤变（CIN）和68例宫颈鳞癌组织替拿素的表达情况，并检测Ki-67标记的增殖指数（PI）和CD34标记的微血管密度（MVD）。结果替拿素从NCE、CIN到宫颈鳞癌组织的阳性表达率分别为0％、8．33％和52．94％，替拿素阳性率有显著升高趋势（P〈0．01）。替拿素在宫颈鳞癌中表达与盆腔淋巴结转移密切相关（P〈0．05），而与国际妇产科联盟（FIGO）分期、组织学分级、间质浸润深度及脉管浸润无关（P〉0．05）。无盆腔淋巴结转移者替拿素表达水平显著高于有盆腔淋巴结转移者（P〈0．05）。替拿素在宫颈鳞癌中表达与MVD正相关（P〈0．05），而与PI无相关性（P〉0．05）。宫颈鳞癌组织替拿素阳性表达者生存率与阴性表达者比较无统计学意义（P〉0．05）。结论宫颈鳞癌组织替拿素过度表达可能促进肿瘤血管生成并抑制盆腔淋巴结转移。

A preliminary study of expression and clinical significance of tenascin protein in cervical squamaus cell carcinoma

Objective To observe the expression and clinical significance of tenascin (TN) protein in cervical squamous cell carcinoma. Methods The expression of TN protein, proliferation index (PI) of cancer cells labeled by Ki-67 protein and microvessel density (MVD) labeled by CD34 protein in 28 cases of normal cervical epithelium (NCE), 36 cases of cervical intraepithelial neoplasm (CIN) and 68 cases of squamous carcinoma of cervix (SCC) were detected by immunohistochemistry SP method. Results The positive rates of expression of TN in NCE, CIN and SCC groups were 0%, 8.33% and 52.94%, respectively. The positive staining reaction of TN increased remarkably from NCE, and CIN to SCC (P>0.01 ). The expression of TN in SCC group was significantly related to pelvic lymph node metastasis (P>0.05), but not related to FIGO staging, histological grading, stroma involvement, and intravascular infiltration ( P>0.05 ). In the cases without pelvic lymph node metastasis, the posi-tive staining reaction of TN in SCC group was significantly higher than that in those without the conditions mentioned above ( P>0.05 ). The expression of TN in SCC group was positively correlated with MVD ( P>0.05 ), but not with PI (P>0.05 ). Compared with the TN negative cases in SCC group, There was not significant difference of patients' survival rate in the TN positive cases ( P>0.05 ). Conclusion The overexpression of TN in SCC group may result in rapid tumor angiogenesis and inhibit cancer cells from pelvic lymph node metastasis.