| 活性氧在密旋链霉菌Act12诱导丹参毛状根中丹参酮积累中的作用 |
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| 引用本文： | 阎岩,赵欣,张顺仓,刘岩,梁宗锁.活性氧在密旋链霉菌Act12诱导丹参毛状根中丹参酮积累中的作用[J].中国中药杂志,2014,39(11):1985-1991. |
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| 作者姓名： | 阎岩 赵欣 张顺仓 刘岩 梁宗锁 |
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| 作者单位： | 西北农林科技大学 生命科学学院, 陕西 杨凌 712100;铜川市中医药发展局, 陕西 铜川 727031;陕西国际商贸学院 医学院, 陕西 咸阳 712046;西北农林科技大学 生命科学学院, 陕西 杨凌 712100;天津天士力现代中药资源有限公司, 天津 300402;西北农林科技大学 生命科学学院, 陕西 杨凌 712100 |
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| 基金项目： | 国家自然科学基金项目（81373908）；陕西省重大产业集群荐项目（2012KTCL02-07） |
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| 摘 要： | 作者前期研究成果显示密旋链霉菌Act12可以上调丹参酮生物合成途径上的关键酶基因的表达大幅提高丹参毛状根中丹参酮含量。该实验则进一步研究了活性氧在Act12诱导丹参毛状根中丹参酮积累的作用。在继代培养21 d的丹参毛状根中添加Act12不同的诱导子及诱导子组合，分别测定不同收获期毛状根的生物量，毛状根中活性氧积累量，丹参酮类成分的积累量和关键酶基因表达量。Act12诱导后丹参毛状根中活性氧含量上升，HMGR和DXR基因表达上调，最高分别达到对照的32.4，4.8倍，毛状根中丹参酮积累增加，最高达到对照的10.2倍；加入活性氧抑制剂CAT和SOD后，丹参毛状根中的活性氧含量较Act12处理显著下降，HMGR和DXR基因表达也明显下降，毛状根中丹参酮含量较Act12处理下降了74.6%。活性氧介导了Act12诱导丹参酮积累，Act12很可能是通过ROS信号通路激活了MVA和MEP途径，从而提高了丹参酮在毛状根中的含量。
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| 关 键 词： | 丹参毛状根 活性氧 密旋链霉菌Act12 次生代谢 3-羟基-3-甲基戊二酰辅酶A还原酶 1-脱氧-D-木酮糖-5磷酸还原异构酶 |
| 收稿时间： | 2013/10/27 0:00:00 |
Roles of reactive oxygen species in Streptomyces pactum Act12-induced tanshinone production in Salvia miltiorrhiza hairy roots |
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| YAN Yan,ZHAO Xin,ZHANG Shun-cang,LIU Yan and LIANG Zong-suo.Roles of reactive oxygen species in Streptomyces pactum Act12-induced tanshinone production in Salvia miltiorrhiza hairy roots[J].China Journal of Chinese Materia Medica,2014,39(11):1985-1991. |
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| Authors: | YAN Yan ZHAO Xin ZHANG Shun-cang LIU Yan and LIANG Zong-suo |
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| Institution: | College of Life Sciences, Northwest Agriculture & Forestry University, Yangling 712100, China;Sun Simiao Institute of Traditional Chinese Medicine, Tongchuan 727031, China;Shaanxi Institute of International Trade & Commerce, Xianyang 712046, China;College of Life Sciences, Northwest Agriculture & Forestry University, Yangling 712100, China;Tianjin Tasly Modern Traditional Chinese Resources Co., Ltd., Tianjin 300402, China;College of Life Sciences, Northwest Agriculture & Forestry University, Yangling 712100, China |
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| Abstract: | Our previous research indicated that the Streptomyces pactum Act12 (Act12) had a certain promotional effect on tanshinone accumulation and up-regulated the expression of genes 3-hydroxy-3-methyglutary1-CoA reductase (HMGR) and 1-deoxy- d-xylulose -5-phosphate reductoisomerase (DXR) in Salvia miltiorrhiza hairy roots. This study focuses on the roles of reactive oxygen species in S. pactum Act12-induced tanshinone production in S. miltiorrhiza hairy roots. The 4% Act12, 4% Act12+CAT and 4% Act12+SOD were added to S. miltiorrhiza hairy root and subcultured for 21 days, the dry weight, contents of reactive oxygen species, contents of tanshinones and expression of HMGR and DXR were determined at different harvest-time. The generation of reactive oxygen species (ROS) in S. miltiorrhiza hairy roots was triggered by 4% Act12 treatment. The relative expressions of genes HMGR and DXR in 4% Act12 treatment were 32.4 and 4.8-fold higher than those in the control. And the total tanshinone in the hairy roots was 10.2 times higher than that of the control. The CAT and SOD could significantly inhibit the ROS accumulation and relative expressions of genes HMGR and DXR in 4% Act12 treatment, which induced the total tanshinone content was decreased by 74.6% comparing with the 4% Act12 treatment. ROS mediated Act12-induced tanshinone production. The Act12 may be via the ROS signal channel to activate the tanshinone biosynthesis pathways. Thereby the tanshinon content in hairy roots was increased. |
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| Keywords: | Salvia miltiorrhiza hairy roots reactive oxygen species Streptomyces pactum Act12 secondary metabolism 3-hydroxy-3-methyglutary1-CoA reductase 1-deoxy-d-xylulose 5-phosphate |
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