新城疫病毒抑制HER 2表达对BGC-823胃癌细胞增殖的影响

Effects of inhibition of HER 2 expression by Newcastle disease virus on the proliferation of human gastric adenocarcinoma cell line BGC-823

Objective:To investigate the effects of selective inhibition of HER 2 expression by Newcastle disease virus to the proliferation of human gastric adenocarcinoma cell line BGC-823.Methods:The expression of HER 2 mRNA and protein in Newcastle disease virus-infected gastric adenocarcinoma cells was determined by RT-PCR and Western blotting.The inhibitory effect of Newcastle disease virus on proliferation of BGC-823 cells were assessed by MTT assay.Apoptotic rate of BGC-823 cells was measured by flow cytometry(FCM).Results:The infection of BGC-823 cells with Newcastle disease virus successfully knocked down the expression of HER 2 at mRNA and protein levels.Down-regulation of HER 2 gene inhibited the proliferation and increased the apoptotic rate of BGC-823 cells compared with the control group(P<0.01).Conclusions:Infection with Newcastle disease virus efficiently killed BGC-823 cells and induced apoptosis.The mechanism may be due to the inhibition of HER 2 gene expression in BGC-823 cells.