低剂量壬基酚促进DU-145细胞增殖及雌激素膜受体GPR30表达的研究

目的:观察低剂量外源性雌激素壬基酚(NP)对人前列腺癌细胞株DU-145增殖和雌激素膜受体GPR30表达的影响。方法:DU-145细胞暴露于不同浓度的NP,通过细胞增殖实验测定DU-145细胞的半数抑制率(IC50),确定低剂量NP的暴露浓度范围;CCK-8实验测定低剂量NP对细胞增殖的影响;RT-PCR法观察DU-145细胞中3种雌激素受体(ER),ER-α、ER-β和雌激素膜受体GPR30的表达水平以及低剂量NP暴露后,雌激素膜受体GPR30表达的变化。结果:NP抑制细胞增殖的IC50为46μmol/l,确定本实验中使用的低剂量NP浓度分别为0.01、0.1、1μmol/l;CCK-8的结果发现,低剂量NP对DU-145细胞具有促增殖作用。RT-PCR的结果发现,DU-145细胞中有3种ER的表达,与前列腺上皮细胞表达类似,低剂量NP具有促GPR30表达的作用。结论:雌激素膜受体GPR30可能在介导低剂量NP促前列腺癌细胞DU-145增殖的过程中起到一定作用。

Low.dose nonylphenol promotes the proliferation of DU-145 cells and expression of membrane estrogen receptor GPR30 in DU.145 cells

Objective： To observe the effects of low-dose exogenous estrogen nonylphenol （NP） on the proliferation of human prostate cancer cell lines DU-145 and the expression of the membrane estrogen receptor GPR30 in the DU-145 cells. Methods ： We exposed DU-145 cells to different concentrations of NP for 24 hours, followed by measurement of the half maximal inhibitory concentration （IC50） of the cells by cell proliferation assay and determination of the concentration of exposure to low-dose NP. We also observed the expressions of 3 estrogen receptors （ER） ,, including ER-α, ER-β and membrane estrogen receptor GPR30, in the DU-145 cells exposed to low-dose NP by RT-PCR. Results ： Cell proliferation assay showed that within a certain range of doses, NP inhibited the proliferation of the DU-145 cells with an IC50 of 46 μmol/L,a much lower dose of NP than ICS0, 0.01,0.1,1 μmol/1 NP, that can promote the proliferation of DU-145 ceils. The results of RT-PCR indicated that the expressions of the three ERs in the DU-145 cells were similar to those in prostate epithelial cells, and that low-dose NP promoted the expression of GPR30. Conclusion： Membrane estrogen receptor GPR30 may play a role in low-dose NP promoting the proliferation of DU-145 cells. Natl J Androl, 2014, 20 （5） ： 405 - 409