宫颈癌细胞中DAPK1基因CpG岛甲基化及其表达

目的 :探讨宫颈癌细胞中凋亡相关蛋白激酶 1(DAPK1 )基因CpG岛甲基化及其表达与宫颈癌的相关性 .方法 :应用甲基化特异性PCR和SABC免疫组化方法 ,检测 32(鳞癌 1 8,腺癌 1 4 )例宫颈癌组织DAPK1基因CpG岛甲基化修饰及蛋白表达 .结果 :宫颈癌中DAPK1基因甲基化扩增阳性率明显增高 5 6 .3% ,与正常宫颈比较有显著性差异 (P <0 .0 5 ) ,鳞癌与腺癌甲基化扩增阳性率无显著差异 (P >0 .0 5 ) ;宫颈癌中DAPK1蛋白表达阳性率降低 1 5 .6 3% ,与正常宫颈比较有显著性差异 (P <0 .0 5 ) ,鳞癌和 7例与腺癌无显著性差异 (P >0 .0 5 ) .结论 :DAPK1基因CpG岛异常甲基化修饰能抑制DAPK1的转录 ,使DAPK1蛋白不表达 ,丧失抑癌作用 ,是促进正常宫颈上皮细胞癌变的一个重要因素

CpG island methylation of DAPK1 and its expression in uterine cervical carcinoma

AIM: To investigate the methylation status of DAPK1 gene and its protein expression in uterine cervical carcinoma and to explore the relationship between them. METHODS: The CpG island methylation of DAPK1 gene was detected by methylation specific PCR (MSP) and the expression of DAPK1 protein was examined with immunohistochemistry in 32 cases of uterine cervical carcinoma, among whom 18 were cases of squamous cell carcinoma and 14 cases of adenocarcinoma. RESULTS: The positive expression rate of MSP in uterine cervical carcinoma was 56.3%, which was significantly higher than that in the normal cervical tissue( P <0.05), but no significant difference was found between squamous cell carcinoma and adenocarcinoma( P >0.05). The expression rate of DAPK1 protein in uterine cervical carcinoma was 15.63%, which was significantly lower than that in the normal cervical tissue( P <0.05), and no significant difference was found between squamous cell carcinoma and adenocarcinoma( P >0.05). CONCLUSION: The hypermethylation of CpG island may play some major role in decreasing DAPK1 protein expression, which is an important factor in the uterine cervical carcinogenesie.