健脾活血方抗酒精性肝损伤脂质过氧化主效应中药分析

目的:探索具有显著抗酒精性肝损伤作用的中药复方健脾活血方抗脂质过氧化的主效应中药,并分析相关作用机制.方法:采用均匀设计法及Lieber-DeCarli乙醇液体饲料诱导的大鼠乙醇性肝损伤模型,以肝组织丙二醛(MDA)含量作为效应指标筛选主效应中药,并再次以该模型验证其药效；进一步检测肝组织超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)、诱导型一氧化氮合酶(iNOS)、谷胱甘肽过氧化物酶(GSH-Px)及血清铁离子(Fe2+)含量以分析主效应中药抗脂质过氧化的机制.结果:含8味中药的健脾活血方中“抗脂质过氧化”主效应药物组合为:葛根、泽泻、五味子、姜黄(剂量比为250∶50∶17∶50),验证及药理分析实验显示:与模型组相比,抗脂质过氧化主效应中药组肝组织MDA,NOS,iNOS及血清Fe2+含量显著降低,SOD,GSH-Px活性显著升高.结论:健脾活血方8味中药中对大鼠乙醇性肝损伤模型“抗脂质过氧化”的主效应中药组合为“葛根+泽泻+五味子+姜黄”,其药理机制在于抑制促氧化物质NOS,iNOS及血清Fe2+产生,增加抗氧化自由基清除酶SOD,GSH-Px活性.

Analysis of Major Effective Compositions in Jianpi Huoxue Decoction against Lipid Peroxidation in Alcoholic Liver Injury

Objective:To investigate the major effective compositions in Jianpi Huoxue decoction against lipid peroxidation which can protect alcohol-induced liver injury remarkably and its possible action mechanism. Method: The rats were given Lieber-DeCarli liquid diet daily to make the alcoholic liver injury model. And Uniform design was adopted to design the experiments. malonyldialdehyde(MDA) in liver tissue was detected as the index. The same mode was used to screen the major herbs and verify the efficacy. The superoxide dismutase(SOD),nitric oxide synthase(NOS),induced nitric oxide synthase(iNOS),glutathione peroxidase(GSH-Px)in liver and Fe²⁺ in serum were detected. Result: Puerariae Lobatae Radix,Alismatis Rhizoma, Schisandrae Chinensis Fructus, and turmeric were the major herbs in Jianpi Huoxue decoction against lipid peroxidation. And the verification experiment showed that compared with the model group, the major herbs could reduce the MDA,NOS,iNOS and Fe²⁺ significantly and promote the activity of SOD,GSH-Px apparently. Conclusion: The combination of Puerariae Radix,Alismatis Rhizoma, Schisandrae Chinensis Fructus, and turmeric were the major effective compositionsof Jianpi Huoxue decoction against lipid peroxidation. The possible action mechanism may lie in inhibiting NOS,iNOS and Fe²⁺ in serum which promote oxidation, and increase the activity of free radical scavenger SOD,GSH-Px which provide anti-oxidation.