| 结核分支杆菌耐多药株katG突变SSCP技术检测的研究 |
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| 引用本文： | 朱中元,陈贻平,陈允凤,王海波,张贵琛,邵寒霜.结核分支杆菌耐多药株katG突变SSCP技术检测的研究[J].海南医学,2000,11(2):76-77. |
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| 作者姓名： | 朱中元 陈贻平 陈允凤 王海波 张贵琛 邵寒霜 |
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| 作者单位： | 1. 海南省农垦总局医院 570311
2. 中国热带农业科学院生物技术国家重点实验室 |
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| 基金项目： | 海南省自然科学基金，39611， |
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| 摘 要： | 目的 建立并评价ＰＣＲ－ＳＳＣＰ检测结核分支杆菌耐药性基因突变的方法。方法 根据ｋａｔＧ基因易变区设计一对引物，ＰＣＲ扩增，产物经沸点断裂成单链，经ＳＤＳ－ＰＡＧＥ，比较电泳的位置。结果 ＰＣＲ检测结核菌ＤＮＡ的灵敏度达到１００个细菌／ｍｌ，与其它细菌和其他分支杆菌无交叉反应。３０株敏感株和Ｈ３７Ｒｖ的ＰＣＲ产物经ＳＳＣＰ检测正常，２０株耐异烟肼结核菌中，１９株的ＰＣＲ产物ＳＳＣＰ检测游异常电泳带
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| 关 键 词： | 结核分支杆菌 基因突变 耐药性 |
| 修稿时间： | 1999年12月12 |
Detection of kat G Mutations of Mycobacterium Tuberculosis Isolates by Means of PCR-SSCP |
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| Zhu Zhongyuan, Chen Yiping, Chen Yunfeng,et al..Detection of kat G Mutations of Mycobacterium Tuberculosis Isolates by Means of PCR-SSCP[J].Hainan Medical Journal,2000,11(2):76-77. |
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| Authors: | Zhu Zhongyuan Chen Yiping Chen Yunfeng |
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| Institution: | Zhu Zhongyuan, Chen Yiping, Chen Yunfeng, et al. |
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| Abstract: | Objective To establish a PCR - SSCP for detection of katG mutations of M. tuberculosis.Method PCR products of katG were denatured and analysed by SDS - PAGE. Result The sensitivity of PCR that we developed was 100 bacteria/ml.There was no cross reaction with other Mycobacteria and bacteria. 30 isolates and H37Rv had no mutation by SSCP. 19 out of 20 isolates which were resistant to INH were found to have katG mutation by SSCP. All 15 isolates which had been found katG mutation by DNA sequencing were obersed to have katG mutation by SSCP. Conclusion The PCR - SSCP we developed can be widely appied to detection of katG mutation. |
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| Keywords: | Mycobacterium tuberculosis Mutation katG SSCP |
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