外周血T淋巴细胞亚群的检测在MARS人工肝治疗前后中的临床意义

目的:探讨检测MARS人工肝治疗前后肝功能衰竭患者外周血T淋巴细胞亚群的临床意义。方法:应用流式细胞技术分别检测健康对照组以及MARS人工肝治疗前后肝功能衰竭患者外周血中T淋巴细胞亚群的绝对计数值,观察其变化规律。结果:32例肝功能衰竭患者外周血中CD3+、CD3+CD4+及CD3+CD8+T淋巴细胞绝对计数值均明显低于正常对照组;CD3+CD4+CD8+T淋巴细胞数明显高于正常对照组。经过MARS人工肝治疗后,患者外周血中CD3+、CD3+CD4+、CD3+CD8+以及CD3+CD4+CD8+T淋巴细胞数均不同程度的升高,但无统计学差异。结论:通过检测MARS人工肝治疗前后肝功能衰竭患者外周血T淋巴细胞亚群,可反映患者机体的免疫状态,对评价MARS人工肝在改善患者免疫功能方面有一定的临床意义。

The clinical value of detecting peripheral blood T lymphocyte subsets in the patients with liver failure treated with MARS

Objective: To identify the clinical value of detecting peripheral blood T lymphocyte and in the patients with liver failure treated with MARS. Methods: To detect peripheral blood T lymphocyte adn in the patients with liver failure treated with MARS and the normal control by flow cytometer , observer the regular pattern was observed. Results: The absolute counts of peripheral blood CD3~+、CD3~+CD4~+ and CD3~+CD8~+ T cells were lower and CD3~+CD4~+CD8~+ T cells were higher in 32 patients with liver failure than in normal control. The counts of CD3~+,CD3~+CD4~+ ,CD3~+CD8~+ and CD3~+CD4~+CD8~+ T cells in the patients treated with MARS were higher that before the with MARS(P>0.05). Conclusion:Detecting peripheral blood T lymphocyte subsets, maiy bean index to reflect the immune state in the patients with liver failure to and is valuable in appraising the function of immune in the patients treated with MARS.