Complete nucleotide sequence of the genome organization of RNA2 of patchouli mild mosaic virus, a new favavirus |
| |
| Authors: | M. Ikegami H. Kawashima T. Natsuaki N. Sugimura |
| |
| Affiliation: | (1) Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan, JP;(2) Department of Plant Pathology, Utsunomiya University, Mine-machi, Utsunomiya, Japan, JP;(3) Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan, JP |
| |
| Abstract: | ![]()
Summary. The complete nucleotide sequence and the genome organization of the RNA 2 of a patchouli mild mosaic virus (PaMMV) was determined. The sequence consists of 3591 nucleotides and contains a single long open reading frame sufficient to code for 118K protein. Three proteins of 52 K, 44 K and 22 K could be encoded by the PaMMV RNA 2 genome. Our analysis of the N-terminal sequences of two species of coat protein (CP) allowed precise location of the CP cistrons within the polyprotein. 44 K and 22 K proteins are the coat proteins. The positions of the cleavage sites are Gln/Ala between 44 K and 22 K coat proteins and Gln/Gly between 52 K and 44 K proteins. Comparison of PaMMV RNA 2 with comoviral and nepoviral RNA 2 showed no sequence similarity. These results as well as previous serological studies strongly suggest that PaMMV is a member in the genus Fabavirus. Received May 29, 1998 Aaccepted July 20, 1998 |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
