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| LC/MS/MS与GC/MS法分析鉴定小鼠尿中沙美特罗的代谢产物 | |
| 引用本文： | 张建丽,徐友宣,邸欣,秦旸,张长久.LC/MS/MS与GC/MS法分析鉴定小鼠尿中沙美特罗的代谢产物[J].中国新药杂志,2004,13(5):424-428. |
| 作者姓名： | 张建丽 徐友宣 邸欣 秦旸 张长久 |
| 作者单位： | 1. 国家体育总局运动医学研究所兴奋剂检测中心,北京,100029;沈阳药科大学药学院分析化学教研室,沈阳,110016 2. 国家体育总局运动医学研究所兴奋剂检测中心,北京,100029 3. 沈阳药科大学药学院分析化学教研室,沈阳,110016 |
| 摘 要： | 目的:鉴定沙美特罗在小鼠尿中的主要代谢产物.方法:ig给药后,收集小鼠尿液,经固相提取,葡萄糖醛酸酶水解,进行LC/MS/MS分析和硅烷化后进行GC/MS分析同时分离鉴定沙美特罗代谢产物.结果和结论:在给药后尿样中发现沙美特罗原型和4种代谢产物M1～M4,其结构推测为19-羟基沙美特罗(M1)、2-羰基沙美特罗(M2)、19-羰基沙美特罗(M3)和19-羟基-8-甲氧基沙美特罗(M4). |
| 关 键 词： | 沙美特罗 代谢产物 |
| 文章编号： | 1003-3734(2004)05-0424-05 |
LC/MS/MS and GC/MS identification of metabolisms of salmeterol in mice urine |
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| ZHANG Jian-li,XU You-xuan,DI Xin,QIN Yang,ZHANG Chang-jiu.LC/MS/MS and GC/MS identification of metabolisms of salmeterol in mice urine[J].Chinese Journal of New Drugs,2004,13(5):424-428. | |
| Authors: | ZHANG Jian-li XU You-xuan DI Xin QIN Yang ZHANG Chang-jiu |
| Abstract: | Objective:To study the metabolites of salmeterol, which has been forbidden by the Medical Commission of the International Olympic Committee(IOC) since 1995. Methods:Urine sam-ples of ten healthy mice were collected within 24h after ig administration of salmeterol. The metabolites of salmeterol(M1-M4) were identified by LC/MS/MS after solid phase extraction followed by enzyme hydrolysis and GC/MS after derivatization. Mass spectrometry was operated in the positive mode for the method of LC/MS/MS. Result and conclusion:Salmeterol and it's four metabolites (M1-M4) were identified both by LC/MS/MS and GC/MS. M1-M4 were deduced as 19-hydroxyl-salmeterol, 2-carbonyl-salmeterol, 19-carbonyl-salmeterol and 19-hydroxyl-8-methoxyl-salmeterol, respectively. |
| Keywords: | LC/MS/MS GC/MS |
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