| 动态比浊法定量测定左卡尼汀氯化钠注射液中细菌内毒素的含量 |
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| 引用本文： | 施春阳,李永进,方建国.动态比浊法定量测定左卡尼汀氯化钠注射液中细菌内毒素的含量[J].中国药房,2004,15(8):499-500. |
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| 作者姓名： | 施春阳 李永进 方建国 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院药学部,武汉市,430030 |
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| 摘 要： | 目的 :应用动态比浊法鲎试验定量测定左卡尼汀氯化钠注射液中的细菌内毒素含量。方法 :将左卡尼汀氯化钠注射液作16倍稀释 ,采用动态比浊法 ,通过干扰试验和验证试验 ,测定其细菌内毒素含量。结果 :将样品作16倍稀释可有效消除对鲎试验的干扰 ,检测其平均回收率均在50 %～200 %范围内。结论 :本法测定左卡尼汀氯化钠注射液中细菌内毒素含量高效、可行
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| 关 键 词： | 动态比浊法鲎试验 左卡尼汀 细菌内毒素 |
| 文章编号： | 1001-0408(2004)08-0499-02 |
| 修稿时间： | 2003年8月24日 |
Application of the Kinetic Turbidimetric Limulus Test to the Endotoxin Assay of Levocarnitine Sodium Chloride Injection |
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| SHI Chunyang,LI Yongjin,FANG Jianguo.Application of the Kinetic Turbidimetric Limulus Test to the Endotoxin Assay of Levocarnitine Sodium Chloride Injection[J].China Pharmacy,2004,15(8):499-500. |
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| Authors: | SHI Chunyang LI Yongjin FANG Jianguo |
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| Abstract: | OBJECTIVE:To apply the kinetic turbidimetric Limulus test to the endotoxin assay of Levocarnitine sodium chloride injection.METHODS:The16-fold dilution of Levocarnitine sodium chloride injection was prepared.The content of bacterial endotoxin in Levocarnitine sodium chloride injection was determined with kinetic turbidimetric Limulus test after screen test and validation test.RESULTS:The16-fold dilution of Levocarnitine sodium chloride injection was effective to eˉliminate the interference in Limulus test.The average recovery was in the range of50%～200%.CONCLUSION:The kinetic turbidimetric Limulus test provides a new and quick method for the quantitative determination of bacterial endotoxin in Levoˉcarnitine sodium chloride injection. |
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| Keywords: | kinetic turbidimetric Limulus test Levocarnitine bacterial endotoxin |
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