桦褐孔菌多糖的提取工艺优化

目的研究桦褐孔菌多糖提取工艺优化。方法以多糖含量为指标，在单因素研究基础上，采用4因素3水平的响应面分析法对桦褐孔菌多糖的提取工艺进行优化研究，并检测重金属砷的含量。结果桦褐孔菌多糖提取的优化工艺条件为：乙醇浓度30％、提取温度95℃、提取时间2．5h、液料比30：1。在该条件下，粗多糖得率为33．6％，样品中多糖含量为6．177％。桦褐孔菌多糖实际提取含量为5．993％，重金属含量降低。结论优化工艺提取率高，快速，重金属含量低，可用于桦褐孔菌的多糖提取。

Optimization of Extraction Technology for Polysaccharides in Inonotus Obliquus

OBJECTIVE To optimize the extraction process for polysaccharides in Inonotus obliquus. METHODS The content ofInonotus obliquus polysaccharides was used as index based on the one-factor tests, and the method of response surface method with 4 factors and 3 levels was adopted. The content of heavy metal in the extraction was detected. RESULTS The optimum conditions of Inonotus obliquus polysaccharides extraction as follows： ethanol concentration was 30%, extraction temperature was 95 ~C, time of extraction was 2.5 h and the ratio of material to solvent was 30 ~ 1. Response surface analysis predicted that the production of crude polysaccharide was 33.6% and the content of polysaccharide was 6.177%, respectively. By using verification test, Inonotus obliquus polysaccharides reached 5.993% without significant difference compared with the prediction. And the level of heavy metal significantly decreased. CONCLUSION The optimized extraction process can obtain high extraction rate, low level of heavy metal content, and can be used for extraction oflnonotus obliquus polysaccharides