HPLC测定心悦胶囊中人参皂苷Rg1、Re及Rb3的含

目的：研究心悦胶囊中人参皂苷Re、人参皂苷豫，及人参皂苷Rb3的定量方法。方法：采用高效液相色谱法。色谱柱：LunC18（250mm×4．6mm，5μm）；流动相：乙腈-水梯度洗脱；检测波长为203nm。结果：线性范围为人参皂苷Rg1在0．0989～1．9780μg（r=0．9991，n=5），人参皂苷Re在0．3120～5．38μg（r=0．9992，n=5），人参皂苷Rb3在0．448—8．96bLg（r=0．9991，n=5）。平均加样回收率人参皂苷Rg，为97．1％，RSD：2．46％（n=6），人参皂苷Re为97．5％，RSD=1．89％（n=6），人参皂苷Rb，为100．0％，RSD=2．28％（n=6）。结论：本法简便、灵敏、准确，可用于心悦胶囊的质量控制。

The Determination of Ginsenoside Rg1 , Ginsenoside Re and Ginsenoside Rb3 in Xinyue Capsules By HPLC

Objective： An assay method was developed for the determination of Ginsenoside Rg1 , Ginsenoside Re and Ginsenoside Rb3 in Xinyue Capsules by HPLC. Methods： The analysis was performed on Lun C~8 （250 mm× 4.6 mm, 5 μm） column. Acetonitrile-water gradient elution, detector wavelength 203 nm ; Results： Ginsenoside Rg1 linear range is 0. 098 9-1. 798 0 μg; relevant coefficient r = 0. 999 1 （n = 5 ） , Ginsenoside Re linear range is 0. 312 0-5.38 μg; relevant coefficient r = 0. 999 2 （ n = 5 ） , Ginsenoside Rgi avevage recovery is 97.1% , RSD =2.46% （ n = 6）. Ginsenoside Re avevage recovery is 97.5%, RSD = 1.89% （ n = 6 ）, Ginsenoside Rb3 linear range is 0. 448-8.96 μg; relevant coefficient r = 0. 999 1 （ n = 5 ）, Ginsenoside Rb3 avevage recovery is 100.0% , RSD =2.28% （n = 6）. Conclusion： The method is simple, rapid and accurate, it is suitable for the quality control of strychnine.