| 基因魔剪CRISPR/Cas:核酸精准检测的新宠和利器 |
| |
| 引用本文： | 冯春凤,王云霞,蒲晓允,熊瑜,刘飞.基因魔剪CRISPR/Cas:核酸精准检测的新宠和利器[J].国际检验医学杂志,2020(3):257-261. |
| |
| 作者姓名： | 冯春凤 王云霞 蒲晓允 熊瑜 刘飞 |
| |
| 作者单位： | 陆军军医大学第二附属医院检验科;陆军军医大学第一附属医院检验科 |
| |
| 基金项目： | 国家自然科学基金项目(81873981);重庆市技术创新与应用示范项目(cstc2018jscx-msybx0060);陆军军医大学第一附属医院医务人员军事医学创新能力提升计划(SWH2018BJLC-05)) |
| |
| 摘 要： | 规则成簇间隔短回文重复序列及其相关蛋白(CRISPR/Cas)系统是存在于大多数细菌及古细菌中的一种获得性免疫系统。作为一种高效的基因定点编辑工具,CRISPR/Cas系统不仅在基因敲出、基因治疗及基因修饰等领域炙手可热,而且正在发展成为核酸精准检测领域的新利器。随着众多研究者的不断探索,便携、快速、低成本、灵敏度高、特异性强的CRISPR/Cas核酸精准检测技术不断涌现。本文就CRISPR/Cas技术在核酸快速精准检测领域的最新成果作一概述,并总结展望其面临的困难和挑战。
|
| 关 键 词： | CRISPR/Cas 核酸 精准检测 |
CRISPR/CAS:a new darlings and powerful tool in the nucleic acid precise detection |
| |
| Institution: | (Department of Clinical Laboratory,the Second Affiliated Hospital of Army Military Medical University,Chongqing 400037,China;Department of Clinical Laboratory,the First Affiliated Hospital of Army Military Medical University,Chongqing 400038,China) |
| |
| Abstract: | Clustered regularly interspaced short palindromic repeats and CRISPR associated proteins(CRISPR/Cas)are an acquired immune system found in most bacteria and archaea.It has been a mature gene editing tool,mainly used in the fields of gene cutting,gene therapy and modification.Recently,researchers have continuously shifted the CRISPR/Cas technology from gene editing to nucleic acid testing,trying to develop a portable,fast,low-cost,high sensitivity and strong specificty nucleic acid detection technology for point-of-care testing.This paper reviews the latest applications of CRISPR/Cas technology in nucleic acid detection and summarizes the potential challenges in the nucleic acids rapidly detecting field. |
| |
| Keywords: | CRISPR/Cas nucleic acid accurate detection |
| 本文献已被 维普 等数据库收录！ |
|
