微弧氧化AZ91D镁合金对成骨细胞早期粘附的影响

目的体外观察探讨新型口腔植入材料微弧氧化(Micro-arc oxidation,MAO)AZ91D镁合金对人成骨样MG63细胞早期粘附的影响。方法实验分为三组:微弧氧化AZ91D镁合金材料实验组A,纯钛材料对照组B和细胞直接生长在培养板对照组C,利用表面轮廓仪、接触角测量仪、扫描电镜(SEM)和能谱分析(EDS)分别测量研究试件表面粗糙度、表面能大小、形貌特点和元素成分;将MG63细胞培养于各组表面,分析比较成骨细胞的早期粘附率及形态学变化。对数据采用SPSS13.0统计软件进行单因素方差分析。结果A组表面为一层粗糙多孔、起伏不平的薄膜,主要元素有Mg、O、Si,粗糙度及表面能均比B组增加(P<0.05);0.5h、1h和2h三个时间点三组细胞粘附率差异有统计学意义(P<0.05),A组>B组>C组;培养4h时A、B两组材料表面成骨细胞形态良好。结论微弧氧化AZ91D镁合金具有良好的成骨细胞相容性,有利于细胞的早期粘附。

Effects of AZ91D magnesium alloy modified by micro-arc oxidation on early adhesion of osteoblast

Objective To investigate the effects of AZ91D magnesium alloy modified by micro-arc oxidation on the early adhesion of human osteoblast-like MG63 cells in vitro. Methods The experiment was divided into 3 groups:magnesium alloy modified by micro-arc oxidation A,pure titanium B and osteoblasts cultured on cell culture plates C.The surface roughness and surface energy of the specimens were measured by the profilometer and the goniometer,SEM and EDS were used to test surface morphology and composition.Then MG63 cells were cultured on the surface of 3 groups,the cell adhesion ratio was counted and the cell morphology was examined.The results were processed by SPSS 13.0 software package for one-way ANOVA. Results The group A produced a layer of porous,rough oxidation coating mainly composed of Mg,O,Si.The surface roughness and surface energy of group A were significantly higher than those of group B(P<0.05).As the cultured time increased,the statistic results indicated that the cell adhesion ratio of group A was significantly higher than that of B and C(P<0.05).SEM observation showed that the adhesion between cells and materials was pretty good after having been cultured for 4h. Conclusions The AZ91D magnesium alloy modified by micro-arc oxidation possesses good cytocompatibility to osteoblast,and it may increase original cell adhesion.