PIM-1蛋白激酶在乳腺癌组织中的表达及意义

目的探讨PIM-1蛋白激酶在乳腺癌组织、乳腺非典型增生组织及正常乳腺组织中的表达及生物学意义。方法利用免疫组织化学、Westernblot方法检测乳腺癌组织、乳腺非典型增生组织和正常乳腺组织中P1M-1蛋白激酶的表达水平。结果乳腺癌组织、乳腺非典型增生组织和正常乳腺组织中PIM-1蛋白激酶的阳性表达率分别为78．75％（63／80）、42．00％（21／50）、23．33％（7／30）；乳腺癌组织中PIM-1的蛋白水平明显高于癌旁正常乳腺组织（灰度值：118．807±19．99VS103．062±13．51，t=8．578，P〈0．05）；PIM-1蛋白激酶的表达与乳腺癌的P-TNM分期和淋巴结转移密切相关（P：0．004，P=0．003），与年龄和肿块大小无关（P=0．654，P=0．084）。结论PIM-1在乳腺癌中表达阳性率明显高于非典型增生的乳腺组织和正常乳腺组织，PIM-1可能是乳腺癌形成过程中的早期分子事件，其表达增强与乳腺癌的浸润性发展密切相关，有可能成为新的早期筛查和判断乳腺癌生物学行为的诊断指标。利用石蜡标本检测指标是可行的，具有方便，易得，经济的特点。

The expression and biological significance of PIM1 protein kinases in breast cancers

Objective . To investigate the expression and biological significance of PIM1 in the breast cancer, atypical hyperplasia breast, and normal breast tissues. Methods The protein expression levels of PIM1 in the breast cancer, atypical hyperplasia breast, and normal breast tissues were assayed by immunohistochemistry and Western blot. Results The expression rates of PIM1 protein were 78.75% （63/ 80）in breast cancers, 42. 00% （21/50）in atypical hyperplasia tissues, and 23.33 % （7/30）in normal breast tissues, respectively. A significant correlation was found between PIM1 expression and the clinical stage and lymphonodus metastasis. However, no significant correlation was found between PIM1 expression and patients＇age and tumor sizes. The level of PIM1 protein in breast cancers was obviously higher than that in non - cancerous tissues. Conclusions The positive rate of PIM1 in breast cancers is significantly higher than that in atypical hyperplasia breast tissues and in normal breast tissues. PIM1 may be an early molecular event in mammary tumorigenesis, and its overexpression may significantly relate to the malignant progres- sion. It would be a new parameter for the early diagnosis and a biomarker for breast cancers. It is feasible to utilize paraffin specimens for index test with advantages of convenience, easy availability, and low expense.