实时定量PCR法分析2型糖尿病患者肠道乳酸杆菌菌种数量的改变

目的:明确2型糖尿病患者与正常对照人群比较是否存在总乳酸杆菌及其具体菌种嗜酸乳杆菌、保加利亚乳杆菌、干酪乳杆菌、植物乳杆菌、鼠李糖乳杆菌数目的差异,以及探索乳酸杆菌与血糖、血脂等代谢指标是否相关.方法:选取2型糖尿病患者50人、正常对照者30人作为研究对象,两组性别、年龄、BMI相匹配.检测糖尿病患者空腹血糖、餐后2h血糖、血脂、C反应蛋白等指标,并收集糖尿病患者及对照者粪便提取细菌总DNA,通过实时荧光定量PCR方法测定目标细菌的拷贝数.结果:2型糖尿病组肠道乳酸杆菌(P<0.001)及嗜酸乳杆菌(P<0.001)、保加利亚乳杆菌(P<0.001)、干酪乳杆菌(P=0.008)、鼠李糖乳杆菌(P<0.001)显著高于对照组,且乳酸杆菌与低密度脂蛋白胆固醇显著负相关(P=0.04).结论:2型糖尿病患者肠道总乳酸杆菌及其某些具体菌种数量增多,乳酸杆菌数量的改变对血脂也存在影响.通过饮食调节肠道乳酸杆菌含量可能有助于改善糖尿病、高脂血症等代谢性疾病.

Real-time quantitative PCR analysis of intestinal Lactobacillus species in type 2 diabetic patients

AIM:To assess the differences in Lactobacillus species between patients with type 2 diabetes mellitus(T2DM) and healthy individuals,and to determine the association between metabolic parameters and Lactobacillus species in T2DM patients.METHODS:This study included 50 T2DM patients and 30 healthy individuals of similar age,gender and BMI.Blood samples were collected from T2DM to measure metabolic parameters.Bacterial DNA was extracted from fecal samples to assess the quantities of bacteria using realtime quantitative PCR with primers specifically targeting V3 region of the 16s rRNA.RESULTS:The quantities of Lactobacillus(P < 0.001),L.acidophilus(P < 0.001),L.bugaricus(P < 0.001),L.cacei(P = 0.008) and L.Rahmnosumand(P < 0.001) were significantly increased in theT2DM group compared to the control group.The quantity of Lactobacillus was significantly negatively associated with LDL cholesterol levels(P = 0.04).CONCLUSION:T2DM patients are characterized by an increase in the quantity of Lactobacillus species in the guts compared to normal controls.Lactobacillus in the guts play a role in influencing cholesterol metabolism in T2DM patients.Modification of the gut microflora by dietary means may help control metabolic diseases such as dyslipidemia and diabetes.