冠心病心脾阳虚证动物模型的建立及附子理中汤的反证效果

目的: 探索冠心病心脾阳虚证动物模型的建立方法并观察附子理中汤的对模型的反证效果。 方法: 100只Wistar大鼠随机分为正常组、模型组、附子理中汤低剂量、中剂量、高剂量组5组，每组20只。模型组采用肩胛骨间棕色脂肪组织（BAT）切除术，术后以高脂饲料喂养、隔日寒冷环境刺激连续21 d，从第22天开始脑垂体后叶素（10 U·kg^-1）皮下注射，每天1次，连续7 d。第29天开始，低剂量、中剂量、高剂量组在模型组基础上，以附子理中汤分别按10，20，40 g·kg^-1 ig每天1次，连续7 d。观察大鼠心功能、磷酸肌酸激酶、胆碱酯酶、甘油三酯、总胆固醇、体重和体温等的变化。 结果: 模型组大鼠有类似于冠心病血脂升高、心肌缺血损伤、心功能不全以及脾阳虚证形寒肢冷、纳少、消瘦、便溏及腹泻表现。与正常组和中剂量、高剂量组比较差异显著（P<0.05）或高度显著（P<0.01）。 结论: 通过复合方式建立病证结合的冠心病心脾阳虚证动物模型是可行的。附子理中汤高剂量能一定程度改善冠心病心脾阳虚证动物模型病理变化。

Establishment of the Animal Model of Coronary Heart Disease due to Heart and Spleen Yang Deficiency Syndrome and Effects of Fuzi Lizhong Decoction Interference

Objective: To explore the method of establishing the animal model of coronary heart disease due to heart and spleen Yang deficiency syndrome and observe the effects of Fuzi Lizhong decoction interference. Method: Hundred Wistar rats were randomly divided into control group,model group,Fuzi Lizhong decoction low-dose group,middle-dose group and high-dose group 5 groups with 20 rats in each group. The model group was applied by incising brown adipose tissue(BAT) then with high fatty diet and cold stimulation every other day for 21 days. From the 22^nd day,the model group was given subcutaneous injection with pitutrin,once a day for 7 days and from the 29^th day,low-dose group,middle-dose group and high-dose group were ig respectively with Fuzi Lizhong decoction (10,20,40 g·kg^-1) based on model group.Once a day for 7 days. To observe the changes of the cardiac function,creatine kinase(CK),cholinesterase(CHE),triglycerides(TG),total cholesterol(TC),rectal temperature and weight. Result: The model rats were familiar to the pathologic change of CHD hyperlipoidemia,the myocardial ischemia damage and the cardiac insufficiency and low rectal temperature,weight lose,diarrhea of spleen Yang deficiency syndrome. There was significant difference or high significant difference(P<0.05 or P<0.01),compared with the control group,middle-dose group and high-dose group. Conclusion: It may provide a valuable method for establishment of combined with disease and Zheng animal model by applying multiple methods during the establishment of the animal model of coronary heart disease due to heart and spleen Yang deficiency syndrome. high-dose Fuzi Lizhong decoction could improve the pathologic changes of model rats to some extent.