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| 旋毛虫肌幼虫核酸疫苗的构建及其在小鼠体内的表达 | |
| 引用本文： | 崔晶,张红卫,王中全,李雍龙.旋毛虫肌幼虫核酸疫苗的构建及其在小鼠体内的表达[J].中国寄生虫学与寄生虫病杂志,2004,22(1):5-8. |
| 作者姓名： | 崔晶 张红卫 王中全 李雍龙 |
| 作者单位： | 1. 华中科技大学同济医学院,武汉,430030;郑州大学医学院寄生虫学教研室,郑州,450052 2. 郑州大学医学院寄生虫学教研室,郑州,450052 3. 华中科技大学同济医学院,武汉,430030 |
| 基金项目： | 河南省教育厅自然科学研究项目 (2 0 0 2 31 0 0 0 1 4 )~~ |
| 摘 要： | 目的 构建和表达旋毛虫肌幼虫编码相对分子质量 (Mr) 31000抗原结构基因 (TspE1)的重组质粒。 方法 通过逆转录 聚合酶链反应 (RT-PCR)特异性扩增TspE1基因 ,构建重组质粒 pUC18-TspE1,并将TspE1基因亚克隆入真核表达载体 pcDNA3中。用基因枪免疫小鼠 ,通过苏木素 伊红 (HE)染色及免疫组化观察重组质粒在皮肤组织内的表达情况。 结果与结论 成功构建 pcDNA3-TspE1重组质粒 ,并在小鼠体内表达。 |
| 关 键 词： | 旋毛虫 核酸疫苗 抗原 基因枪技术 真核表达 |
| 文章编号： | 1000-7423(2004)-01-0005-04 |
Construction of DNA Vaccine of Trichinella spiralis Muscle Larvae and Its Expression in Mice |
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| CUI Jing,ZHANG Hong-wei,WANG Zhong-quan,LI Yong-long.Construction of DNA Vaccine of Trichinella spiralis Muscle Larvae and Its Expression in Mice[J].Chinese Journal of Parasitology and Parasitic Diseases,2004,22(1):5-8. | |
| Authors: | CUI Jing ZHANG Hong-wei WANG Zhong-quan LI Yong-long |
| Institution: | Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. |
| Abstract: | Objective To construct and express recombinant plasmid containing the structural gene encoding {Mr 31 000} antigen of Trichinella spiralis muscle larvae. MethodsThe target gene TspE1 was amplified by RT_PCR, cloned into pUC18 vector, and sub_cloned into the eukaryotic expression vector pcDNA3. BALB/c mice were immunized with the purified recombinant plasmid pcDNA3_TspE1 by gene_gun bombardment. The expression of recombinant plasmid in the skin tissue was observed by HE staining and immunohistochemical staining. Results and Conclusion The recombinant plasmid pcDNA3_TspE1 was successfully constructed and expressed in the BALB/c mice. |
| Keywords: | Trichinella spiralis DNA vaccine antigens gene_gun technique eukaryotic expression |
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