TRPM7通过PI3K/AKT/ERK信号途径影响脑胶质瘤细胞增殖、上皮-间质转化

目的 探讨沉默M型顺时受体电位通道7(melastatin transient receptor potential channel 7,TRPM7)对脑胶质瘤细胞增殖、上皮-间质转化的影响及其作用机制.方法 采用qRT-PCR和Western blot检测TRPM7在人正常星形胶质HA1800细胞株以及脑胶质瘤细胞株...

TRPM7 affects the proliferation and epithelial-mesenchymal transition of brain glioma cells through PI3K/AKT/ERK signaling pathway

Objective To investigate the effect of silent melastatin transient receptor potential channel 7(TRPM7)on the proliferation and epithelial-mesenchymal transition of brain glioma cells and the underlying mechanism.Methods The TRPM7 expression in human normal astrocyte cell line HA1800 and glioma cell lines(U251,U87 and U373)were detected by qRT-PCR and Western blot.U251 cells were divided into the U251 group(untransfected U251 cells),U251/shNC group and U251/shTRPM7 group,then the expression of TRPM7 was detected by q RT-PCR and Western blot;the cell proliferation ability was detected by cell clone formation assay;the cell migration and invasion ability were detected by Transwell migration and invasion experiments;the expression of E-cadherin and Vimentin was detected by cellular immunofluorescence assay;the expression of PI3 K/AKT/ERK signaling pathway-related proteins were detected by Western blot.Results Compared with HA1800 cells,TRPM7 was highly expressed in glioma cell lines(P<0.05).Silencing TRPM7 reduced the expression of TRPM7 in U251 cells(P<0.001),and the ability of cell proliferation,migration and invasion were significantly reduced(P<0.01);E-cadherin expression was increased(P<0.001)and Vimentin expression was decreased(P<0.01);the expression level of PI3 K protein was down-regulated(P<0.01),and the phosphorylation level of AKT protein and ERK1/2 protein were also significantly reduced(P<0.01).Conclusion Silencing TRPM7 can inhibit the proliferation,migration,invasion ability and epithelial-mesenchymal transformation of U251 cells,which may be related to the PI3 K/AKT/ERK signaling pathway.