非霍奇金淋巴瘤患者行外周血单个核细胞采集前后血细胞的变化及影响因素分析

目的 探索拟行CAR-T治疗的非霍奇金淋巴瘤患者以及健康供者使用血细胞分离机采集单个核细胞后血细胞的变化,探讨影响终产品单个核细胞数量的因素以更好的提高采集效率.方法 使用COM.TEC血细胞分离机采集23例非霍奇金淋巴瘤患者以及22例健康供者的单个核细胞,分别于采集前,采集后即刻抽取血样,检测红细胞(RBC)、白细胞...

Analysis of the Changes and Influencing Factors of Blood Cells before and after the Collection of Mononuclear Cells in Patients with Non-Hodgkin's Lymphoma

Objective To improve collecting efficiency,we investigated the changes of blood cells and influencing factors of collecting mononuclear cells from peripheral blood before CAR-T treatment.Methods MNC cells were collected from 23 Non Hodgkin's lymphoma patients and 22 healthy donors by using COM.TEC blood cell separator.Routine blood test was performed before and after MNC collection,including red blood cell(RBC),white blood cell(WBC),lymphocyte cell,mononuclear cell and platelet count.The changes of blood cells before and after collection were analyzed.At the same time,the age,height,weight,body mass index(BMI),collection time,white membrane pumping volume,single collection volume,circulation times,single circulation volume,total circulation volume and the final product mononuclear cell count(MNC)were analyzed by multiple linear regression.Results The blood cell count of healthy donors and non-Hodgkin's lymphoma patients decreased after MNC collection(all P<0.05);Multiple linear regression analysis showed that the pre-collection MNC,single collection volume and cycle times were closely related to the final product MNC(all P<0.05).However,age,height,weight,BMI,systemic circulation blood volume,and flow rate,had no significant correlation with the final product MNC(all P>0.05).Conclusions In order to ensure the health level of patients and healthy donors,we should make individual collection plan for different recipients.The volume of single collection,the number of cycles and the count of pre-collection monocytes cells are the main factors for the efficiency of MNC collection.