12种香豆素类化合物抑制鼻咽癌活性的筛选

目的探讨12种香豆素类化合物体外抑制鼻咽癌细胞的活性。方法利用CCK-8法测定不同浓度的12种香豆素类化合物对鼻咽癌细胞CNE-2和HONE-1体外抑制生长的活性,利用两点法求算对应的半数抑制浓度（Half maximal inhibitory concentration,IC50）。随后选定1、10、100μmol/L浓度的蛇床子素及秦皮乙素分别处理两鼻咽癌细胞,利用CCK-8法绘制5 d的生长曲线,并观察其对克隆形成能力的影响。结果在CNE-2细胞中,蛇床子素、秦皮乙素和佛手柑内酯的IC50分别是（44.8±5.2）μmol/L、（129.6±8.9）μmol/L及（143.7±9.7）μmol/L;在HONE-1细胞中的IC50分别是（38.6±6.3）μmol/L、（62.7±7.0）μmol/L及（73.4±7.2）μmol/L。蛇床子素和秦皮乙素在选定的浓度下均对鼻咽癌细胞的生长曲线产生持续抑制的效果,并能显著抑制鼻咽癌细胞的克隆形成能力（P〈0.01）。结论蛇床子素、秦皮乙素和佛手柑内酯具有具有较为显著的体外抑制鼻咽癌细胞活性。

Screening of Twelve Coumarins Antitumor Activity Nasopharyngeal Carcinoma

Objective To investigate the effect of coumarins antitumor activity in nasopharyngeal carcinoma（ NPC） cells in vitro. Methods The inhibition were testing by CCK-8,when the NPC cells＇ CNE-2 and HONE-1 exposed to coumarins for48 h,and 1,10,100 μmol /L concentration were selected to 5 d growth curve experiment and clone formation experiment for osthole and esculetin. Results In CNE-2 cells,the IC50 of Osthole,Esculetin,Bergapten were（ 44. 8 ± 5. 2） μmol /L,（129.6 ±8.9） μmol/L and（143.7 ±9.7） μmol/L. In HONE-1 cells,the IC50 of Osthole,Esculetin,Bergapten were（38.6 ±6.3） μmol/L,（62.7 ±7.0） μmol/L and（73.4 ±7.2） μmol/L. The Osthole and Esculetin inhibited CNE-2and HONE-1 growth and clone formation in each concentration. Conclusions These results highlight that osthole and esculetin exert antiproliferative effects in NPC.