| 碱性成纤维细胞生长因子对染铅大鼠海马神经元胞浆ERK的保护作用 |
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| 引用本文： | 吴攀峰,;焦铭,;孙慧荣,;吴基良.碱性成纤维细胞生长因子对染铅大鼠海马神经元胞浆ERK的保护作用[J].咸宁医学院学报,2014(6):461-462. |
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| 作者姓名： | 吴攀峰 ;焦铭 ;孙慧荣 ;吴基良 |
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| 作者单位： | [1]武汉大学基础医学院药理系,湖北武汉430071; [2]湖北科技学院糖尿病心脑血管病变湖北省重点实验室;,湖北武汉430071; [3]武汉大学附属中南医院心内科,湖北武汉430071; |
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| 基金项目： | 湖北科技学院糖尿病专项基金项目（ZX1006） |
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| 摘 要： | 目的研究碱性成纤维细胞生长因子（b FGF）对染铅大鼠海马神经元细胞外调节蛋白激酶（ERK）的保护作用。方法神经元培养7 d后,用0.6μg/L的b FGF以及不同浓度铅处理细胞,然后测定ERK活力。结果铅处理神经元30 min后,在铅浓度0.1~2.0μmol/L时ERK活性明显升高,其中2.0μmol/L时最高。经过b FGF处理以后的细胞,在铅浓度1.0~4.0μmol/L时ERK活性与对照组相比无明显差异。结论染铅浓度不同对ERK活性影响也不同,b FGF可以保护神经元中ERK活性。
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| 关 键 词： | 碱性成纤维细胞生长因子 铅 海马神经元 细胞外调节蛋白激酶 |
Protection of bFGF on ERK in Cytosol of Hippocampal Neuron Exposed to Lead |
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| Institution: | WU Pan-feng,JIAO Ming,WU Ji-liang,et al ( Department of Pharmacology ,School of Basic Medical Sciences, Wuhan University, Wuhan Hubei 430071 ,China) |
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| Abstract: | Objective To study the protection of basic fibroblast growth factor（ b FGF） on the activity of extracellular signal-regulated kinase（ ERK） in hippocampal neuron of rats exposed to lead. Methods After cultured for 7 days,the hippocampal neuron was treated with 0. 6 μg / L b FGF and lead with different concentration. The activity of ERK was then determined. Results The activity of ERK was increased when treated with0. 1 ~ 2. 0 μmol / L lead for 30 min. While treated with b FGF in advance,the activity of ERK had no significant difference compared with the control group. Conclusion The activity of ERK was changed by treated with different concentrations of lead,and b FGF can protect ERK activity in neuron. |
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| Keywords: | Basic fibroblast growth factor Lead Hippocampal neuron Extracellular signal-regulated kinase |
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