塞来昔布对前列腺癌细胞株PC-3中VEGF-C及bcl-2 mRNA表达的影响

 目的探讨环氧化酶-2(COX-2)选择性抑制剂塞来昔布(celecoxib)对前列腺癌细胞株PC-3中VEGF-CmRNA及bcl-2mRNA表达的影响,研究COX-2抑制剂抗肿瘤的作用机制。方法将培养的PC-3细胞分为四组:试验组(分别以10μmol/L、20μmol/L、40μmol/L不同浓度塞来昔布处理)和对照组,采用RT-PCR的方法检测四组中VEGF-C及bcl-2mRNA的表达。结果10μmol/L塞来昔布组VEGF-C/GAPDH值及bcl-2/GAPDH值与对照组相比差别均无统计学意义(P>0.05);20μmol/L及40μmol/L塞来昔布组VEGF-C/GAPDH值分别为0.370±0.063、0.263±0.062,bcl-2/GAPDH值分别为0.339±0.047、0.272±0.042,两组中上述两项指标均较对照组明显下降(P均<0.01)。结论塞来昔布可能通过对前列腺癌细胞株PC-3中VEGF-C及bcl-2mRNA表达的抑制发挥其抗肿瘤作用。

Effects of Celecoxib on Expression of VEGF-C and bcl-2 mRNA in Prostate Cancer PC-3 Cell Lines

Objective To investigate effects of cyclooxygenase-2 selective inhibitor on the expression of VEGF-C and bcl-2 mRNA in human prostate cancer PC-3 cell lines,and study the anticancer mechanism of COX-2 selective inhibitor.Methods The PC-3 cells were divided to four groups:Test groups(The cells were treated with celecoxib at different concentrations-10 μmol/L,20 μmol/L,40 μmol/L) and control group(celecoxib was not used),detected the expression of VEGF-C and bcl-2 mRNA in the four groups by RT-PCR method.Results 　There were no statistical difference of the VEGF2C/ GAPDH andbcl22/ GAPDH value between 10 μmol/ L celecoxib group and cont rol group ( P > 0. 05) ;VEGF2C/ GAP2DH value in 20μmol/ L and 40μmol/ L celecoxib groups were 0. 370 ±0. 063 and 0. 263 ±0. 062 respec2tively , bcl22/ GAPDH value in both the groups above were 0. 339 ±0. 047 and 0. 272 ±0. 042 respective2ly , both the value above in the two groups decreased significantly compared with cont rol group ( P <0. 01) . Conclusion 　Celecoxib maybe plays it s antitumor effect by inhibiting the expression of VEGF2Cand bcl22 mRNA in prostate cancer PC23 cell lines.