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| Determination of anti-endomysium IgA antibodies in the diagnosis of celiac disease： Comparison of a novel ELISA-based assay with conventional immunofluorescence | |
| 引用本文： | Poland DC,Ceelie H,Dinkelaar RB,Beijer C. Determination of anti-endomysium IgA antibodies in the diagnosis of celiac disease： Comparison of a novel ELISA-based assay with conventional immunofluorescence[J]. World journal of gastroenterology : WJG, 2006, 12(17): 2779-2780. DOI: 10.3748/wjg.v12.i17.2779 |
| 作者姓名： | Poland DC Ceelie H Dinkelaar RB Beijer C |
| 作者单位： | Department of Clinical Chemistry Diaconessenhuis Leiden,Netherlands,Department of Clinical Chemistry Albert Schweitzer Ziekenhuis,Dordrecht,Netherlands,Department of Clinical Chemistry Albert Schweitzer Ziekenhuis,Dordrecht,Netherlands,Department of Clinical Chemistry Diaconessenhuis,Leiden,Netherlands |
| 摘 要： | AIM: To evaluate the novel anti-endomysium (anti-EMA) detection based on ELISA. METHODS: Anti-EMA IgA was measured by a novel ELISA in 196 patients with gastrointestinal symptoms and suspected mal-absorption. Data were compared with those obtained by the conventional IF test. RESULTS: A good concordance of 98% was found between these two assays. In sera of 161 patients (82%) both assays tested negative whereas in sera of 31 patients (16%) both assays tested positive for the presence of anti-EMA antibodies. Discrepancies between EMAELISA and EMA-immunofluorescence (IF) were found in only 4 patients (2%). CONCLUSION: This ELISA can replace IF for the detection of anti-EMA antibodies and provide clinicians with an excellent tool to screen for celiac disease in patients with gastrointestinal complaints. |
| 关 键 词： | 肌内膜 IgA抗体 腹腔疾病 免疫荧光 |
| 收稿时间： | 2005-11-24 |
Determination of anti-endomysium IgA antibodies in the diagnosis of celiac disease: comparison of a novel ELISA-based assay with conventional immunofluorescence |
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| Poland Dennis C W,Ceelie Huib,Dinkelaar Rob B,Beijer Cornelis. Determination of anti-endomysium IgA antibodies in the diagnosis of celiac disease: comparison of a novel ELISA-based assay with conventional immunofluorescence[J]. World journal of gastroenterology : WJG, 2006, 12(17): 2779-2780. DOI: 10.3748/wjg.v12.i17.2779 | |
| Authors: | Poland Dennis C W Ceelie Huib Dinkelaar Rob B Beijer Cornelis |
| Affiliation: | 1. Department of Clinical Chemistry, Diaconessenhuis, Leiden, Netherlands 2. Department of Clinical Chemistry, Albert Schweitzer Ziekenhuis, Dordrecht, Netherlands |
| Abstract: | AIM: To evaluate the novel anti-endomysium (anti-EMA) detection based on ELISA. METHODS: Anti-EMA IgA was measured by a novel ELISA in 196 patients with gastrointestinal symptoms and suspected mal-absorption. Data were compared with those obtained by the conventional IF test. RESULTS: A good concordance of 98% was found between these two assays. In sera of 161 patients (82%) both assays tested negative whereas in sera of 31 patients (16%) both assays tested positive for the presence of anti-EMA antibodies. Discrepancies between EMAELISA and EMA-immunofluorescence (IF) were found in only 4 patients (2%). CONCLUSION: This ELISA can replace IF for the detection of anti-EMA antibodies and provide clinicians with an excellent tool to screen for celiac disease in patients with gastrointestinal complaints. |
| Keywords: | Celiac disease Endomysium ELISA Immunofluorescence Histology |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |