|
|||||
|
|
| 水通道蛋白4在高原脑水肿大鼠脑组织中的表达变化及意义 | |
| 引用本文： | 梁林,杨术旺,龚海英. 水通道蛋白4在高原脑水肿大鼠脑组织中的表达变化及意义[J]. 武警医学院学报, 2011, 20(12): 932-934,937,F0003 |
| 作者姓名： | 梁林 杨术旺 龚海英 |
| 作者单位： | 1. 武警后勤学院科研部成果处 天津300162 2. 武警后勤学院训练部研究生处 天津300162 3. 武警后勤学院药物化学教研室 天津300162 |
| 基金项目： | 武警后勤学院院级科研基金项目（WYM201011） |
| 摘 要： | [目的]探讨水通道蛋白4(aquaporin4,AQP4)与高原脑水肿之间的关系.[方法]制作大鼠高原缺氧损伤模型,测脑组织含水量,采用RT-PCR、免疫组织化学、Western blotting技术测定脑组织缺氧损伤24 h后AQP4 mRNA和蛋白表达变化.[结果]缺氧损伤后,脑组织含水量增加,水肿明显；HE染色显示部分神经元肿胀或浓缩；缺氧24 h后,AQP4 mRNA和蛋白表达量均明显增加(P＜0.05).[结论]AQP4在高原缺氧脑水肿的形成过程中可能发挥了重要作用. |
| 关 键 词： | 水通道蛋白4 大鼠 高原缺氧 脑水肿 |
Expression of AQP4 in cerebral edema rats in high altitude |
|
| LIANG Lin , YANG Shu-wang , GONG Hai-ying. Expression of AQP4 in cerebral edema rats in high altitude[J]. Acta Academiae Medicinae CPAPF, 2011, 20(12): 932-934,937,F0003 | |
| Authors: | LIANG Lin YANG Shu-wang GONG Hai-ying |
| Affiliation: | (Department of Scientific Research,Logistics University of Chinese People＇s Armed Police Force,Tianjin 300162,China) |
| Abstract: | 【Objective】To study the relationship between AQP4 and cerebral edema in high altitude.【Methods】The model of cerebral edema in high altitude was established.The content of water in brain was measured.The expression of AQP4 mRNA and protein were measured by RT-PCR,immunohistochemical and Western blotting.【Results】The water content increased and the edema was evident.HE staining showed neuron edema and condensation.The expression of AQP4 mRNA and protein were up-regulated obviously after 24 h of hypoxia（P 0.05）.【Conclusion】The results shows that AQP4 might play an important role in the formation of high altitude cerebral edema. |
| Keywords: | Aquaporin 4 Rat Plateau hypoxia Brain edema |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |