| 低抗凝肝素对大鼠肝星状细胞生长的影响 |
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| 引用本文： | 李文才,张锦生,朱虹光,张秀荣,张月娥. 低抗凝肝素对大鼠肝星状细胞生长的影响[J]. 郑州大学学报(医学版), 2007, 42(3): 451-453 |
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| 作者姓名： | 李文才 张锦生 朱虹光 张秀荣 张月娥 |
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| 作者单位： | 1. 郑州大学第一附属医院病理科,郑州大学基础医学院病理学教研室,河南省肿瘤病理重点实验室,郑州,450052;复旦大学上海医学院病理学教研室,上海,200032
2. 复旦大学上海医学院病理学教研室,上海,200032 |
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| 摘 要： | 目的:探讨低抗凝肝素对胎牛血清培养的大鼠肝星状细胞生长的影响.方法:肝星状细胞经体积分数为0.4%胎牛血清/DMEM培养液同步化48 h后分为对照组和实验组,对照组以体积分数为10%胎牛血清处理,实验组分别以体积分数为10%胎牛血清和不同质量浓度(8 g/L,40 g/L,200 g/L,1 000 g/L,5 000 g/L)低抗凝肝素处理,应用MTT法测定其对肝星状细胞生长的影响.结果:低抗凝肝素(40～5 000 g/L)对肝星状细胞的增殖有明显抑制作用(P＜0.05).在不同的作用时间,低抗凝肝素(200 g/L)均对肝星状细胞生长有明显抑制作用,且在其作用48 h后肝星状细胞生长速度明显减慢.结论:低抗凝肝素对大鼠肝星状细胞生长具有抑制作用.
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| 关 键 词： | 大鼠 肝星状细胞 低抗凝肝素 细胞生长 |
| 收稿时间： | 2006-08-24 |
| 修稿时间： | 2006-08-24 |
Effect of low anticoagulative activity heparin on the growth of rat hepatic stellate cells |
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| LI Wencai,ZHANG Jinsheng,ZHU Hongguang,ZHANG Xiurong,ZHANG Yue'e. Effect of low anticoagulative activity heparin on the growth of rat hepatic stellate cells[J]. Journal of Zhengzhou University: Med Sci, 2007, 42(3): 451-453 |
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| Authors: | LI Wencai ZHANG Jinsheng ZHU Hongguang ZHANG Xiurong ZHANG Yue'e |
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| Affiliation: | 1 Department of Pathology, the First Affiliated Hospital, Zhengzhou University ; Department of Pathology, College of Basic Medical Sciences;Henan Key Laboratory for Tumor Pathology, Zhengzhou 450052 ;2 Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032 |
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| Abstract: | Aim: To explore the effect of low anticoagulative activity heparin on the growth of rat hepatic stellate cells(HSCs). Methods:Rat HSCs were treated with 0.4% fetal calf serum (FCS)/DMEM for 48 hours and divided into the control group and experiment groups. HSCs in the control group were treated with 10% FCS, and HSCs in the experiment groups were treated with 10% FCS and low anticoagulative activity heparin with different concentrations ( 8 g/L, 40 g/L, 200 g/L, 1 000 g/L, 5 000 g/L). MTT reduction assay was used to evaluate the effect of low anticoagulative activity heparin on the growth of rat HSCs. Results: Low anticoagulative activity heparin with different concentrations (40~5 000 g/L) all inhibited the growth of HSCs significantly. At different acting time points, low anticoagulative activity heparin (200 g/L) could inhibit the growth of HSCs significantly, especially after 48 hours. Conclusion: Low anticoagulative activity heparin can inhibit the growth of rat HSCs. |
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| Keywords: | rat hepatic stellate cell low anticoagulative activity heparin cell growth |
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